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PonceauSisusedforthedetectionofproteinsonPVDFandnitrocellulosemembraneswithalimitofdetectionofabout250ngoftransferredprotein.Thedyerapidlystainsproteinsonmembranespinkorlightredforeasyvisualinspectionofproteintransfer. ThestainingisreversIBLeandcompatiblewithsubsequentWesternblotting.PonceauSSolutioncontains0.1%PonceauS(w/v)and5%aceticacid.
References
- AnalyticalChemistry(2016),doi:10.1021/acs.analchem.6b00699