One-StepLumitein™UVProteinGelStainisaready-to-useluminescentproteingelstain.GelsstainedwithOne-StepLumitein™UVProteinGelStaincanbevisualizedwithavarietyofdifferentUV-basedfluorescenceimagingsystems.One-StepLumitein™UVgelstainingrequiresonlyasingle5-30minutestainingstepwithoutfixation.Destainingisoptional.Moreover,One-StepLumitein™UVProteinGelStainofferssaferhandlingandeaseofdisposal,becauseitisanaqueous-basedsolutionthatdoesnotcontainhazardousmethanoloraceticacid.One-StepstainsarecertifiedunderCCRTitle22asnon-toxictotheenvironmentfordraindisposalafterasimplepHneutralizationstep.FurThermore,One-StepLumitein™UVcostssignificantlylessthanotherfluorescentproteingelstains.One-StepLumitein™UVProteinGelStainisavailablein1literbottleor4literCubitainer®sizes.
One-StepLumitein™UVcandetectaslittleasapproximately1-10ngofproteinperbanddependingonthestainingmethodused,althoughstainingintensityvariesbetweenproteins.TheresultsobtainedwithOne-StepLumitein™UVarecomparabletothosewithOriole™FluorescentGelStain;however,One-StepLumitein™UVhasamoreconvenientprotocol,doesnotcontainhazardoussolvents,anddoesnotcausegelshrinkage.StainingisfullycompatIBLewithmassspectrometryandEdman-basedsequencing.
BiotiumalsooffersOne-StepLumitein™ProteinGelStain,whichcanbeimagedusingaUVtransIlluminator,visiblelightgelimager,orlaser-basedgelscanner(suchasaTyphoon®scanner).One-StepLumitein™UVgivesabettersignalonaUVboxthanOneStepLumitein™,butisnotcompatiblewithabluelightilluminatororlaser-basedscanner.
BiotiumalsooffersOne-StepBlue™ProteinGelStain,arapid,easy-to-use,non-toxicalternativetoCoomassiestainingforvisibleblueproteinstainingandoptionalnear-infraredfluorescence-basedgelimaging.
Emailustorequestafreesample!
References
n/a
