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Biotin-16-dUTPcanbeenzymaticallyincorporatedintoDNAvianicktranslation,randompriming,or3′endterminallabeling.Theterminaldeoxynucleotidyltransferase(TdT)-mediatedbiotin-dUTPnickend-labeling(TUNEL)methodhasbeencommonlyusedforapoptosisstudies.Thenumber’16’isthenumberofatomsinthelinkerbetweenbiotinanddUTP.Biotiumalsooffersbiotin-11-dUTP(catalogno.40029-1)andbiotin-20-dUTP(catalogno.40030-1).Thelengthofthelinkeraffectstheincorporationefficiencyofthebiotin-dUTPprobeintoDNAusingDNApolymerases,anditalsoaffectsbiotin/avidinorbiotin/streptavidin.Ingeneral,theshorterthelinker,themoreefficientlythebiotin-dUTPisincorporatedintoDNAbyDNApolymerases.Ontheotherhand,alongerlinkershouldfacilitateinteractionbetweenbiotinandavidinorstreptavidin.
- Lyophilizedsolidsuitableforlongtermstorage;containslyophilizedTEbuffer
- Storeat-20°C
- C32H48N7O18P3Sli4
- MW:971.5
Biotin-16-dUTPisalsoavailableasa 1mMsolutioninpH7.5Tris-HClbuffer(catalogno.40022).
References
1.JournalofVirologicalMethods(2013),http://dx.doi.org/doi:10.1016/j.jviromet.2013.12.019
2.TheOpenGenomicsJournal5,18-29(2012)