TheAccuGreen™HighSensitivitydsDNAQuantitationSolutionis designedforusewithhandheldfluorometerssuchastheQubit®fluorometerfromThermoFisher.TheAccuGreen™assayisspecificfordsDNA,andcanquantifyDNAsamplesintherangeof.01-10ng/uL. Forhigh-sensitivityDNAquantitationusingafluorescentplatereader,werecommendusingourAccuClear™orAccuBlue™NextGendsDNAQuantitationKits,whichprovideunprecedentedsensitivity,accuracyandwidelinearrange.
TheAccuGreen™HighSensitivitydsDNAQuantitationSolutionincludes AccuGreen™dyeandassaybuffer. BiotiumalsoofferstheAccuGreen™HighSensitivitydsDNAQuantitationKit(catalogno.31066),whichincludesdye,buffer,andasetof0and10ng/uL calfthymusdsDNAstandards,foruseintheQubit® pre-programmeddsDNAHighSensitivityassay.
Features
- OptimizedforusewiththeQubit®Fluorometer
- Usesthepre-programmed Qubit® dsDNAHighSensitivityassay setting
- Linearrangeinassay:0.1-100ngdsDNA
- Greenfluorescence(Ex/Em502/523nm)
Components
- AccuGreen™Dye,200X
- AccuGreen™Buffer,1X
Kitsizes
- The500assaykit(31068)containsenoughreagentstoperform1,250reactions,includingstandards(allowingthemeasurementofapproximately500DNAsamples)
- The100assaytrialkit(31068-T)containsenoughreagentstoperform250reactions,includingstandards(allowingthemeasurementofapproximately100DNAsamples)
OtherDNAQuantitationKits:
| dsDNAQuantitationKit | Lineardetectionrange(dsDNAinassay) | Ex/Em(nm) | Features |
|---|---|---|---|
| AccuBlue™NextGen | 1pg*-3ng | 468/507 | Quantitationofextremelypreciousordilutesamples.OptimalforsensitiveapplicationssuchasNGSordigitalPCR.*Limitofdetectionintherangeof1pgto5pgdependingoninstrumentsensitivity. |
| AccuClear™UltraHighSensitivity | 30pg-250ng | 468/507 | Thiskitoffersgreatsensitivityandthewidestlinearrange. |
| AccuBlue™HighSensitivity | 0.2-100ng | 485/530 | QuantitationofdsDNAfrom0.2-100ng,withasafer,membraneimpermeableDNAdye. |
| AccuGreen™HighSensitivity | 0.1-100ng | 502/523 | QuantitationofDNAusingtheQubit®fluorometerfromThermoFisher. |
| AccuBlue™BroadRange | 2-2000ng | 350/460 | ForquantitationofhighconcentrationDNAinabroadlinearrangewithbluefluorescence.Assaycanbeextendedto4000ngwithminorlossoflinearity. |
References
n/a
