GelRed™isanultrasensitive,extremelystableandenvironmentallysafefluorescentnucleicaciddyedesignedtoreplacethehighlytoxicethidiumbromide(EB)forstainingdsDNA,ssDNAorRNAinagarosegelsorpolyacrylamidegels.GelRed™isfarmoresensitivethanEBwithoutrequiringadestainingstep.GelRed™andEBhavevirtuallythesamespectra,soyoucandirectlyreplaceEBwithGelRed™withoutchangingyourexistingimagingsystem.
GelRed™canbeusedtostaindsDNA,ssDNAorRNAinagarosegelsviaeitherprecastorpostgelstaining.GelRed™canalsobeusedtostaindsDNA,ssDNAorRNAinpolyacrylamidegelsviapostgelstaining.GelRed™isalsocompatIBLewithdownstreamDNAmanipulationssuchasrestrictiondigest,sequencing,andcloning.Fordetailedprotocolsforuse,pleasedownloadtheGelRed™ProductInformationSheet.AlsoseeourGelRed™andGelGreen™FrequentlyAskedQuestions(FAQs).
AseriesofsafetytestshaveconfirmedthatGelRed™isnoncytotoxic,nonmutagenicandnonhazardousatconcentrationswellabovetheworkingconcentrationsusedingelstaining.Asaresult,workingstrengthGelRed™canbesafelydisposedofdownthedrainorinregulartrash,providingconvenienceandreducingcostinwastedisposal.Fordetailedtestresults,youmaydownloadtheGelRed™/GelGreen™SafetyReport.
GelRed™3Xinwater(catalogno.41001)isready-to-useforpost-electrophoresisgelstaining,andissuppliedina4Lcubitainer.BiotiumalsooffersGelRed™10,000Xinwater(catalogno.41003)andGelRed™10,000XinDMSO(catalogno.41002).GelRed™inwaterisanewer,saferformulationandourrecommendedformat.WecontinuetoofferGelRed™inDMSOforestablisheduserswhodonotwishtoaltertheirprotocols.
AlsoseeGelGreen™NucleicAcidGelStain,asaferreplacementforSYBR®gelstains,whichiscompatiblewithvisiblelightexcitation.
FEATURES
- SaferthanEB:ShownbytheAmestestandotherteststobenonmutagenicandnoncytotoxic
- Easydisposal:Passedenvironmentalsafetytestsfordirectdisposaldownthedrainorinregulartrash
- Ultra-sensitive:MuchmoresensitivethanEtBrandSYBR®Safe
- Extremelystable:Availableinwater,stableatroomtemperatureforlong-termstorageandmicrowavable
- Simpletouse:Verysimpleproceduresforprecastorpost-electrophoresisgelstaining
- CompatiblewithastandardUVtransIlluminator:ReplacesEtBrwithnoopticalsettingchange
- Compatiblewithdownstreamapplications:Compatiblewithgelpurification,restrictiondigest,sequencingandcloning
PleasealsoseeourEvaGreen®dye,abreakthroughnucleicaciddyeideallysuitedforquantitativereal-timePCR(qPCR).Byincorporatingasmart“release-on-demand”DNA-bindingtechnology,EvaGreen™haslowPCRinhibitionwhileexhibitingsuperiorsensitivity.SimilartoGelRed™,EvaGreen™isnon-toxic,non-mutagenic,andremarkablystable.
GelRedanditsusesarecoveredbygrantedand/orpendingUSandInternationalpatents.SYBRistrademarkofMolecularprobes,Inc.
References
1.EurBiophysJ(2014),doi:10.1007/s00249-014-0995-4
DownloadalistofselectedReferencesforGelRedandGelGreen.
