AccuClear™UltraHighSensitivitydsDNAQuantitationSolutionutilizesanovel,next-generationDNAbindingdyeforhighlysensitiveandaccuratequantitationofpurifieddsDNAsamplesacrossabroadrangeofDNAconcentrations.Thisassayhasa linearrangebetween0.03ngand250ngofdsDNAperassayinmicroplateformat,makingitthemostversatileDNAquantitationassay.Unlikeabsorbance-basedmeasurements,AccuClear™dyeishighlyselectivefordouble-strandedDNAoversinglestrandedDNAorRNA.
TheAccuClear™dsDNAquantitationassayisdesignedforusewithfluorescence96-wellplatereadersequippedwithexcitationandemissionfiltersfordetectinggreenfluorescence.TheuniquespectralpropertiesofAccuClear™dyemakeitespeciallywell-suitedforusewithinstrumentswithblueLEDexcitationsources.AccuClear™alsoiscompatIBLewithhandheldfluorometerssuchasInvitrogen’squbit®andPromega’sQuantiFluor®-P,howeverthestandardcurvecalibrationprogramsfortheseinstrumentsmaynotcoverthefulldynamicrangeoftheAccuClear™kitstandardcurve.ForDNAquantitationusingtheQubit®werecommendourAccuGreen™HighSensitivitydsDNAQuantitationKit(catalogno.31066),whichwasformulatedspecificallyforthatinstrument.
TheAccuClear™UltraHighSensitivitydsDNAQuantiationKitwith1DNAStandardcontainsAccuClear™dye,20Xassaybuffer,anda25ng/uLcalfthymusdsDNAstandardstocksolutionthatcanbeusedtoprepareasetofstandards.BiotiumalsoofferstheAccuClear™UltraHighSensitivitydsDNAQuantiationKitwith7DNAStandards(1000assays,catalogno.31028),whichincludesAccuClear™dye,1Xassaybuffer,andasetofpredilutedcalfthymusdsDNAstandards.AccuClear™UltraHighSensitivitydsDNAQuantitationSolution(1000assays,catalogno.31027),whichincludesdyeand1Xbufferwithoutstandards,isavailableforcustomerswhowishtopreparetheirownDNAstandards.
Components
- AccuClear™Dye(100X)
- AccuClear™Buffer(20X)
- CalfThymusdsDNAStandard,25ng/uL
Features
- Linearrange:0.03-250ngdsDNA
- Greenfluorescence(Ex/Em460/507nm)
- Unrivaledsensitivityanddynamicrange
- NovelgreenfluorescentdyeisaperfectmatchforblueLEDexcitationsources
- Greenfluorescencedetectioncompatiblewithfluorescencemicroplatereader,handheldfluorometer,orNanodrop®fluorospectrophotometer.
OtherDNAQuantitationKits:
| dsDNAQuantitationKit | Lineardetectionrange(dsDNAinassay) | Ex/Em(nm) | Features |
|---|---|---|---|
| AccuBlue™NextGen | 1pg*-3ng | 468/507 | Quantitationofextremelypreciousordilutesamples.OptimalforsensitiveapplicationssuchasNGSordigitalPCR.*Limitofdetectionintherangeof1pgto5pgdependingoninstrumentsensitivity. |
| AccuClear™UltraHighSensitivity | 30pg-250ng | 468/507 | Thiskitoffersgreatsensitivityandthewidestlinearrange. |
| AccuBlue™HighSensitivity | 0.2-100ng | 485/530 | QuantitationofdsDNAfrom0.2-100ng,withasafer,membraneimpermeableDNAdye. |
| AccuGreen™HighSensitivity | 0.1-100ng | 502/523 | QuantitationofDNAusingtheQubit®fluorometerfromThermoFisher. |
| AccuBlue™BroadRange | 2-2000ng | 350/460 | ForquantitationofhighconcentrationDNAinabroadlinearrangewithbluefluorescence.Assaycanbeextendedto4000ngwithminorlossoflinearity. |
AccuClearkitsareintendedforquantitationofpurifieddsDNAsamples.Forcellquantitationassays,pleaseseeourcellviABIlityandcytotoxicityassays.
AccuClear™technologyiscoveredbygrantedU.S.andinternationalpatents.
References
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