5-Aminoallyl-dUTP(AA-dUTP)canbeenzymaticallyincorporatedintoDNA.Theresultingamine-containingDNAcanbesubsequentlylabeledwithafluorescentdye,biotinorotherhaptensviaconventionalpeptidecouplingmethod(1,2).Thistwo-stepmethodforlabelingnucleicacidsisconsiderablymoreeconomicalthantheone-stepmethodusingaprelabeleddUTP.
- 10mMsolutioninTEbuffer
- Storeat-20°C
- C12H17N3Na3O14P3
- MW:589.17
AA-dUTPisalsoavailableinlyophilizedform(catalogno.40020-1).
References
1.BiolChemHoppeSeyler371,953(1990).
2.Biotechniques28,518(2000).