TheYeastLive-or-Dye™FixableLive/DeadStainingKitisafixable,fluorescentassaytodetermineyeastviABIlity.Thekitcontainstwofluorescentcomponents,ThiazoleOrangeandLive-or-Dye™568/583.

TheYeastLive-or-Dye™FixableLive/DeadStainingKitisafixable,fluorescentassaytodetermineyeastviability.Thekitcontainstwofluorescentcomponents,ThiazoleOrangeandLive-or-Dye™568/583.

Live-or-Dye™568/583isafixablereddye,designedfordiscriminationbetweenliveanddeadcellsduringflowcytometryormicroscopy.Live-or-Dye™568/583isacellmembraneimpermeableamine-reactivedye.Itisabletoenterintodeadcellsthathavecompromisedmembraneintegrityandcovalentlylabelfreeaminesonintracellularproteins.Thisdyelabelingisextremelystable,allowingthecellstobefixedandpermeabilizedwithoutlossoffluorescenceordyetransferbetweencells.

ThiazoleOrangeisagreenfluorescentdyethatlocalizestothecytoplasm,withsomenuclearaccumulation.Itisfixable,resultingindiffusecytoplasmicstaininginfixedcells.

Afterstainingyeastwiththiskit,bothlivinganddeadcellswillbestainedwithgreenfluorescence(Ex/Em512/533nm).Onlydeadcellswillbestainedwithredfluorescence(Ex/Em568/583nm),andmayappearyellowwhenthetwoimagesareoverlaid.

KitComponents(for1000assays):

• Live-or-Dye™568/583(solid),4vials
• ThiazoleOrange10mMinDMSO,100uL
• AnhydrousDMSO,250uL

您买的GelRed是真的吗？ 我们都知道EB很毒，但是您知道AO（吖啶橙）比EB更毒吗？ AO（吖啶橙）也是能透过细胞膜完全嵌入细胞核DNA的。 正品GelRed的分子量在400以上，现在市面上大部分的假货、山寨货通过质谱分析分子量在200多，可以确定含有AO（EB的分子量在80几，AO的分子量是200以上） 为了您的身体健康，请从正规渠道购买正品，认准二维码防伪标，扫一扫，查真伪