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WheatgermagglutininbindsspecificallytoN-acetylglucosamineinthepeptidoglycanlayerofgram-positivebacteria.TheLiveBacterialGramStainKitcontainswheatgermagglutinin(WGA)conjugatedtoCF™594tostainthesurfaceofgram-positivecellswithredfluorescence.CF™594isspectrallysimilartoTexasRed®,butwithbrighterfluorescence.ThekitalsoincludesDAPItostaintheDNAofallbacteriawithbluefluorescence.Gram-positivebacteriastainedusingthiskitwillshowafluorescentblueinteriorandfluorescentredcellsurface,whilegram-negativebacterialwillbestainedwithbluefluorescenceonly.
References
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