WheatgermagglutininbindsspecificallytoN-acetylglucosamineinthepeptidoglycanlayerofgram-positivebacteria.TheLiveBacterialGramStainKitcontainswheatgermagglutinin(WGA)conjugatedtoCF™594tostainthesurfaceofgram-positivecellswithredfluorescence.CF™594isspectrallysimilartoTexasRed®,butwithbrighterfluorescence.ThekitalsoincludesDAPItostaintheDNAofallbacteriawithbluefluorescence.Gram-positivebacteriastainedusingthiskitwillshowafluorescentblueinteriorandfluorescentredcellsurface,whilegram-negativebacterialwillbestainedwithbluefluorescenceonly.
References
n/a