TheBacterialViabilityandGramStainKitcontainsthreecomponents:CF™488A-WGA,DAPI,andEthD-IIIsolutionsfordistinguishingbetweengram-negativeandgram-positive,aswellasliveversusdeadbacteria.CF™488A-labeledwheatgermagglutininstainstheN-acetylglucosamineofthepeptidoglycanlayerofgram-positivebacteria1withgreenfluorescence(Ex/Em490/515nm).Thebacterialviabilitycomponentoftheassayisbasedontheintegrityofthecellmembrane.EthD-IIIisanucleicacidbindingdyethatismembraneimpermeable,whichselectivelystainscellswithcompromisedplasmamembraneswithredfluorescence(Ex/Em530/620nm).AllcellsstainbluewiththemembranepermeableDNAdyeDAPI(Ex/Em358/461nm).Onekitissufficient200assayswhenusedaccordingtothekitprotocol.
TheBacterialViabilityandGramStainKithasbeentestedonthefollowingbacterialspecies:Bacillussubtilissubsp.subtilis,Escherichiacoli,Micrococcusluteus,Pseudomonasfluorescens,andStaphylococcusepidermidis.
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