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FDP(fluoresceindiphosphate,tetraammoniumsalt,highpurity)isthemostsensitivefluorogenicalkalinephosphatasesubstrate.Itisalsoanexcellentsubstrateforproteintyrosinephosphatase(PTPase)andthusthesubstrateofchoiceinthehighthroughputscreening(HTS)forPTPaseinhibitor.FDPitselfiscolorless,buttheendproductoftheenzymaticreactionisthegreenfluorescentdyefluorescein.
- λEx/λEm=490/514nm(endproduct)
- ε=90,000(endproduct)
- Off-whitesolidsolubleinwater
- Storeat-20°Candprotectfromlight,especiallyinsolution
- C20H26N4O11P2
- MW:560.39
- [217305-49-2]
References
1.ApplEnvironMicrobiol64,1526(1998).
2.BiolRes27,241(1994).
3.AnalBiochem285,58(2000).
4.JBiomolScreen4,327(1999).
5.ProcNatlAcadSciUSA93,3068(1996).