NucView™530Caspase-3Substrate,1mMinPBS,providesaconvenienttoolfordetectingapoptosisinintactcellsbasedoncaspase-3/7activityusingeitherconfocalmicroscopyorflowcytometry.
Incontrasttootherfluorogeniccaspasesubstratesorfluorescentcaspaseinhibitorbased(FLICA)assays,NucView™caspase-3substratescanbeusedtodetectcaspase-3/7activitywithinindividualintactcellswithoutinhibitingapoptosisprogression.NucView™substratesconsistofafluorogenicDNAdyecoupledtothecaspase-3/7DEVDrecognitionsequence.Thesubstrate,whichisinitiallynon-fluorescent,penetratestheplasmamembraneandentersthecytoplasm.Inapoptoticcells,caspase-3/7cleavesthesubstrate,releasingthehigh-affinityDNAdye,whichmigratestothecellnucleusandstainsDNAwithfluorescence.Thus,NucView™Caspase-3Substratesarebifunctional,allowingdetectionofcaspase-3/7activityandvisualizationofmorphologicalchangesinthenucleusduringapoptosis.Thestainingisalsoformaldehyde-fixable.
NucView™530Caspase-3Substratestainsapoptoticcellnucleiwithorangefluorescence(Ex/Em528/563nm,Figure1),fordetectionintheCy®3channelbyfluorescencemicroscopy(Figure2)orthePEchannelbyflowcytometry(Figure3).NucView™530canbeusedformulti-colorimagingwithblue,green,orfar-redfluorescentprobes.Notethatwhenexcitedbythe488nmlaserline,NucView™530alsofluorescesintheFITCchannel,andthereforecannotbeanalyzedtogetherwithgreenprobesbyflowcytometry.
NucView™530Caspase-3Substrateisavailableasa1mMsolutioninDMSO(catalogno.10406)orPBS(phosphate-bufferedsaline).ThesubstrateinPBSisformulatedforuseincellsthataresensitivetoDMSOtoxicity.Innon-DMSOsensitivecelltypes,addingDMSOduringthesubstrateincubationmayenhanceNucView™530staining.
Biotiumalsooffersbluefluorogenic NucView™405Caspase-3Substrate,andgreenfluorogenicNucView™488Caspase-3Substrateandkits.
CydyeisaregisteredtrademarkofGEHealthcare.
您买的GelRed是真的吗？ 我们都知道EB很毒，但是您知道AO（吖啶橙）比EB更毒吗？ AO（吖啶橙）也是能透过细胞膜完全嵌入细胞核DNA的。 正品GelRed的分子量在400以上，现在市面上大部分的假货、山寨货通过质谱分析分子量在200多，可以确定含有AO（EB的分子量在80几，AO的分子量是200以上） 为了您的身体健康，请从正规渠道购买正品，认准二维码防伪标，扫一扫，查真伪
