NucView™488Caspase-3Substrateisanovelcellmembrane-permeablefluorogeniccaspasesubstratedesignedfordetectingcaspase-3/7activitywithinlivecellsinrealtime.Visit ourProductVideopage toseeavideoofNucView™488inaction.
Unlikeconventionalcaspaseassays,NucView™488Caspase-3Substratedetectscaspase-3/7activitywithinindividualintactcellswithoutinhibitingcaspaseactivity.ThesubstrateconsistsofafluorogenicDNAdyeandaDEVDsubstratemoietyspecificforcaspase-3/7.Thesubstrate,whichisbothnon-fluorescentandnonfunctionalasaDNAdye,rapidlycrossescellmembranestoenterthecytoplasm,whereitiscleavedbycaspase-3toformahigh-affinityDNAdyethatstainsthenucleusbrightgreen.Thus,theNucView™488Caspase-3Substrateisbi-functional,allowingdetectionofintracellularcaspase-3/7activityandvisualizationofchangesinnuclearmorphologyduringapoptosis.Thefluorescentstainingproducedinresponsetocaspase-3/7activityiscompatIBLewithsubsequentfixationandpermeABIlizationforimmunostaining.
NucView™ 488Caspase-3Substrate,isalsoofferedasa1mMsolutioninPBS(phosphate-bufferedsaline)(catalogno.10403)foruseincellsthataresensitivetoDMSOtoxicity.Innon-DMSOsensitivecelltypes,addingDMSOduringthesubstrateincubationmayenhanceNucView488staining.Inadditiontostand-alonesubstratestocksolutions,weoffera NucView™488assaykit withdetailedapplicationprotocols.BiotiumalsooffersthebluefluorescentNucView™405Caspase-3Substrateforusewiththe405nmlaserlineandtheorangefluorescentNucView™530fordetectionintheCy3®channel.
DownloadtheNucView™Flyerformoreinformation.
NucView™enzymesubstratetechnologyiscoveredbyU.S.patents.
References
Cen,etal.DEVD-NucView488:anovelclassofenzymesubstratesforreal-timedetectionofcaspase-3activityinlivecells.TheFASEBJournalpublishedonlineFebruary8,2008.
Downloadafulllistofreferences:ValidatedcelllinesforNucView488withreferences
