Biotium/NucView 488 and RedDot 2 Apoptosis and Necrosis Kit/30072/100-assays

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¥6160.00
货号:30072
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品牌:Biotium
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商品描述
NucView™488andRedDot™2Apoptosis/NecrosisAssayKitcontainsNucView™488Caspase-3Substratefordetectionofcaspase-3/7activity.Thefar-reddead-cellstainRedDot™2isincludedforstainingnecroticandlateapoptoticcellsthathavecompromisedplasmamembraneintegrity.

NucView™488andRedDot™2Apoptosis/NecrosisAssayKitcontainsNucView™488Caspase-3Substratefordetectionofcaspase-3/7activity.Thefar-reddead-cellstainRedDot™2isincludedforstainingnecroticandlateapoptoticcellsthathavecompromisedplasmamembraneintegrity.Thiskitprovidesaconvenienttoolforprofilingapoptoticandnecroticcellpopulationsbyfluorescencemicroscopyorflowcytometry.

Incontrasttootherfluorogeniccaspasesubstratesorfluorescentcaspaseinhibitorbased(FLICA)assays,NucView™488Caspase-3Substratecanbeusedtodetectcaspase-3/7activitywithinindividualintactcellswithoutinhibitingapoptosisprogression.ThesubstrateconsistsofafluorogenicDNAdyecoupledtothecaspase-3/7DEVDrecognitionsequence.Thesubstrate,whichisinitiallynon-fluorescent,penetratestheplasmamembraneandentersthecytoplasm.Inapoptoticcells,caspase-3/7cleavesthesubstrate,releasingthehigh-affinityDNAdye,whichmigratestothecellnucleusandstainsDNAwithbrightgreenfluorescence.Thus,NucView™488Caspase-3Substrateallowsdetectioncaspase-3/7activityandvisualizationofmorphologicalchangesinthenucleusduringapoptosis.

RedDot™2isacellmembrane-impermeable,far-reddyewithhighselectivityformembranecompromisedordeadcells.RedDot™2hasfar-redemissionat695nm,well-separatedfromthegreenfluorescenceofNucView™488. TheexcitationmaximumofRedDot™2is665nm,butitcanbeefficientlyexcitedbywavelengthsfrom488to647nm,andthereforecanbeusedwiththe488nmflowcytometrylaserline.

Note:whileNucView™488stainingisformaldehyde-fixableandcompatIBLewithsubsequentimmunostaining,fixationafterstainingwithRedDot™2isnotrecommendedbecauseitcanresultinincreasedbackgroundstainingofhealthycells.

SeeourfullselectionofNucView™substratesandkits.

NucView™enzymesubstratetechnologyiscoveredbyU.S.patents.

 

References

Cen,etal.DEVD-NucView488:anovelclassofenzymesubstratesforreal-timedetectionofcaspase-3activityinlivecells.TheFASEBJournalpublishedonlineFebruary8,2008.

Downloadafulllistofreferences:ValidatedcelllinesforNucView488withreferences

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