D-Luciferinisasubstratefortheenzymefireflyluciferase.Chemiluminescenceat560nmisgeneratedwhenluciferinisoxidizedbyanATP-dependentprocess,catalyzedbyluciferase.Luciferaseisencodedbythelucgene,whichiswidelyusedasareportergeneinavarietyofcells.Becauseoftheintrinsiclowbackgroundofchemiluminescencetechnique,detectionofthelucgeneexpressioncanbemadeatverylowlevel.Inaddition,luciferin/luciferasehasbeenusedtomeasure10-15molarquantityofATP.D-luciferin,potassiumsaltisawater-solubleformofD-luciferin.
- Lightyellowsolidsolubleinwater
- Storeat-20°Candprotectfromlight
- C11H7KN2O3S2
- Mwt:318.4
- [115144-35-9]
References
1.ApplOpt.50(10),1389(2011).
2.PLoSONE5(10),e13545(2010).
3.AntioxidantsandRedoxSignaling(2010)doi:10.1089/ars.2010.3203
4.Biomaterials31,1429(2010).
5.MethodsinMolecularBIOLOGy(2010)DOI10.1007/978-1-60761-901-7_11
6.PlantPhysiology139,1557(2005).
7.CurrentBiology15,1352(2005).
8.CancerResearch64,6684(2004).
9.ThePlantCell16,769(2004).
10.PNAS(2003)doi:10.1073/pnas.2435285100
