Thiskitprovidesatwo-colorfluorescencestainingonbothlivebacteria(green)anddeadbacteria(red)usingtwoprobesDMAOandEthD-III.DMAOisagreen-fluorescentnucleicaciddyewhichstainsbothliveanddeadbacteria.EthD-IIIisared-fluorescentnucleicaciddyethatselectively stainsdeadbacteriawithdamagedcellmembranes.WithanappropriatemixtureofDMAOandEthD-III,bacteriawithintactcellmembranesshow greenfluorescence,whereasbacteriawithdamagedcellmembranesshowredfluorescence.Thekitissuitableforusewithfluorescencemicroscopesandflowcytometers.Theassayprinciplesaregeneralandapplicabletomostbacteriatypes.For100flowcytometryassaysor1000microscopyassays.
KitComponents:
- 2vials(100uL/vial):DMAO,5mM
- 2vials(150uL/vial):EthidiumHomodimer-III(EthD-III),2mM
Features:
- DualDetection:Detectliveanddeadbacteriacellsinacellpopulationsimultaneously
- Simple&Fast:15mindyeloADIngandmeasurewithoutwashing
- Economic:PerformviABIlityandcytotoxicityassaysatthesametime
- Versatile:AnalysiscompatIBLewithflowcytometersandfluorescencemicroscopesusingpopularsettingsforfluoresceinandpropidiumiodide.
References
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