TheViABIlity/CytotoxicityAssayKitforAnimalLive&DeadCellsprovidesgreen/redfluorescentstainingofliveanddeadcells,respectively.Theassayemploystwoprobesthatdetectintracellularesteraseactivityinlivecellsandcompromisedplasmamembraneintegrityindeadcells.

TheViability/CytotoxicityAssayKitforAnimalLive&DeadCellsprovidesgreen/redfluorescentstainingofliveanddeadcells,respectively.Theassayemploystwoprobesthatdetectintracellularesteraseactivityinlivecellsandcompromisedplasmamembraneintegrityindeadcells.TheesterasesubstratecalceinAMstainslivecellsgreen,whilethemembrane-impermeableDNAdyeEthidiumhomodimerIII(EthD-III)stainsdeadcellsred.Thekitissuitablefordetectionusingfluorescencemicroscopy,fluorescencemicroplatereader,orflowcytometry.Theassayprinciplesaregeneralandapplicabletomosteukaryoticcelltypes,includingadherentcellsandcertaintissues,butnottobacteriaoryeast.Thisfluorescence-basedmethodofassessingcellviabilitycanbeusedinplaceoftrypanblueexclusion,⁵¹Crrelease,andsimilarmethodsfordeterminingcellviabilityandcytotoxicity.EthD-III(#40050and#40051)isaproprietaryDNAstaindevelopedbyBiotiumtobeasuperioralternativetothespectrallysimilardyeEthidiumhomodimerI(EthD-I).EthD-IIIhashigherDNAbindingaffinityandthusbrighterfluorescence(30%brighterthanEthD-I).

SpectralProperties
Calcein(endproductafterhydrolysisofcalceinAM):
Ex/Em:494/517nm(pH8)

EthidiumhomodimerIII(EthDIII):
Ex/Em:530/620nm(withDNA)

您买的GelRed是真的吗？ 我们都知道EB很毒，但是您知道AO（吖啶橙）比EB更毒吗？ AO（吖啶橙）也是能透过细胞膜完全嵌入细胞核DNA的。 正品GelRed的分子量在400以上，现在市面上大部分的假货、山寨货通过质谱分析分子量在200多，可以确定含有AO（EB的分子量在80几，AO的分子量是200以上） 为了您的身体健康，请从正规渠道购买正品，认准二维码防伪标，扫一扫，查真伪