Biotium’slineofLive-or-Dye™FixableViABIlityStainingKitsaredesignedfordiscriminationbetweenliveanddeadcellsduringflowcytometryormicroscopy. Live/deadstainsareusefulprobestoincludewhenanalyzingcellsurfaceproteinexpressionbyflowcytometry,becausetheyallowintracellularfluorescencesignalfromdeadcellswithpermeableplasmamembranestobeexcludedfromanalysis. Inmicroscopy,live/deadstainsallowunambiguousvisualdiscriminationofdeadcells.
Live-or-DyeNucFix™Redisaunique,cellmembraneimpermeabledyethatspecifically stainsthenucleiofdeadcells.Thedyeisabletoenterintodeadcellsthathavecompromisedmembraneintegrityandcovalentlylabelthecellnucleus,allowingforcleardifferentiationofliveanddeadcellsbyeithermicroscopyorflowcytometry.UnlikeothercommonlyusednuclearstainssuchasPropidiumIodideorDRAQ7,NucFix labelingisextremelystable,allowingthecellstobefixedandpermeabilizedwithoutlossoffluorescenceordyetransferbetweencells.TheLive-or-Dye™NucFixRedstainingprotocolhasbeenoptimizedtomaximizelive/deaddiscriminationwithminimallivecellstaining,inordertopreventinterferencewithimmunostaining.
Kitcontents:
50assaytrialsize:
- 1viallyophilizeddye(50labelingreactions)
- AnhydrousDMSOfordyereconstitution
200assaysize:
- 4vialslyophilizeddye(50labelingreactionspervial)
- AnhydrousDMSOfordyereconstitution
λEx/λEm=520/610nm
References
n/a
