Fireflyluciferaseiswidelyusedasareporterforstudyinggeneregulationandfunction,andforpharmaceuticalscreening.Biotium’sSteady-Luc™HTSassaysystem isahomogeneousfireflyluciferasereportergeneassaykitwithahalf-lifeofabout3hoursforthequantificationoffireflyluciferaseexpressioninmammaliancells,allowingbatchprocessinginmicroplates.Theassaybufferisprovidedinlyophilizedformatforconvenientroomtemperatureshippingand-20°Cstorage.

Fireflyluciferaseiswidelyusedasareporterforstudyinggeneregulationandfunction,andforpharmaceuticalscreening.Itisaverysensitivegeneticreporterduetothelackofendogenousactivityinmammaliancellsortissues.However,thelightproductionresultingfromthereactionleadstoformationofsuicidaladenyl-oxyluciferinattheenzymesurface.Itresultsinveryshorthalf-lifeofthelightemissionwithaflash-typekinetics.Becauseofthis,flash-typeluminescencemustbedetectedimmediatelyafterinitiatingthereaction,whichisnotcompatIBLewithbatchanalysisofsamples.

Biotium’sSteady-Luc™HTSassaysystemisaproprietarymixtureofsubstancesthatmodifytheenzymaticreactiontoproducealonglastingsignal(steadyglow)bypreventingtheformationofadenyl-oxyluciferinattheenzymesurface.Itisahomogeneoushighsensitivityfireflyluciferasereportergeneassaykitwithahalf-lifeofabout3hoursforthequantificationoffireflyluciferaseexpressioninmammaliancells,allowingbatchprocessinginmicroplates.

Glow-typeluciferaseassayslikeSteady-Luc™havelowerluminescencesignalcomparedtoflash-typeassays.Thesensitivityandlimitofdetectionoftheassaywilldependonluciferaseexpressionlevelsinyourexperimentalsystemaswellasluminometersensitivity.

TheSteady-Luc™FireflyHTSAssayKit(Lyophilized)iscomparabletoouroriginalSteady-Luc™HTSAssay(catalogno.30028),butwithassaybufferprovidedinlyophilizedformatforeconomicalshippingandconvenientstorage.Simplydissolvethelyophilizedassaybufferinthesuppliedreconstitutionbufferbeforeuse.

KitComponents:

• D-Luciferin
• Steady-LucFireflyAssayBuffer(Lyophilized)
• ReconstitutionBuffer

Features:

• Simple:singlestephomogeneousassay.
• Glowkinetics:luminescencehalflifeofabout3hours.
• Convenient:lyophilizedassaybufferforconvenientshippingandstorage.
• Economical:saveupto45%comparedtootherbrands.

MoreLuciferaseKits

Catalognumber	Product	Description
30085	FireflyLuciferaseAssayKit2.0	Flash-typefireflyluciferaseassaykit.
30075	FireflyLuciferaseAssayKit(Lyophilized)	Fireflyluciferaseassaykitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C.
99923	5XFireflyLysisBuffer	ExtralysisbufferforusewithFireflyLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well.
30028	Steady-Luc™FireflyHTSAssayKit	Glow-typefireflyluciferaseassaywithsignalhalf-lifeofabout3hoursforhigh-throughputscreeninginmulti-wellplates.
30028L	Steady-LucFireflyHTSAssayKit(Lyophilized)	Steady-Luckitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C.
30082	RenillaLuciferaseAssayKit2.0	Flash-typeRenillaluciferaseassaykit.
99912	5XPassiveLysisBuffer	ExtralysisbufferforusewithRenillaLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well.
30081	FireflyandRenillaLuciferaseSingleTubeAssayKit	AssaykitformeasuringfireflyandRenillaactivitiessequentiallyinthesamesampleinasingletube.
99821-100mL	1XPassiveLysisBuffer2.0	ExtralysisbufferforusewithFirefly&RenillaSingleTubeAssayKits,onlyrequiredifusingwellsizeslargerthan24-well.
30020	ATP-Glo™BioluminometricCellViABIlityAssayKit	Flash-typeluminescence-basedassayformeasuringcellviabilityorATPconcentration.
22003	MiniCellScrapers	Mini-cellscrapers,usefulforlysingcellsin96-well,48-well,or24-wellplates.

您买的GelRed是真的吗？ 我们都知道EB很毒，但是您知道AO（吖啶橙）比EB更毒吗？ AO（吖啶橙）也是能透过细胞膜完全嵌入细胞核DNA的。 正品GelRed的分子量在400以上，现在市面上大部分的假货、山寨货通过质谱分析分子量在200多，可以确定含有AO（EB的分子量在80几，AO的分子量是200以上） 为了您的身体健康，请从正规渠道购买正品，认准二维码防伪标，扫一扫，查真伪