Biotium/Steady-Luc Firefly HTS Assay Kit/30028-T/40-assays

价格
¥800.00
货号:30028-T
浏览量:127
品牌:Biotium
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商品描述
Fireflyluciferaseiswidelyusedasareporterforstudyinggeneregulationandfunction,andforpharmaceuticalscreening.Biotium’sSteady-Luc™HTSassaysystem isahomogeneousfireflyluciferasereportergeneassaykitwithahalf-lifeofabout3hoursforthequantificationoffireflyluciferaseexpressioninmammaliancells,allowingbatchprocessinginmicroplates.

Fireflyluciferaseiswidelyusedasareporterforstudyinggeneregulationandfunction,andforpharmaceuticalscreening.Itisaverysensitivegeneticreporterduetothelackofendogenousactivityinmammaliancellsortissues.However,thelightproductionresultingfromthereactionleadstoformationofsuicidaladenyl-oxyluciferinattheenzymesurface.Itresultsinveryshorthalf-lifeofthelightemissionwithaflash-typekinetics.Becauseofthis,flash-typeluminescencemustbedetectedimmediatelyafterinitiatingthereaction,whichisnotcompatIBLewithbatchanalysisofsamples.

Biotium’sSteady-Luc™HTSassaysystemisaproprietarymixtureofsubstancesthatmodifytheenzymaticreactiontoproducealonglastingsignal(steadyglow)bypreventingtheformationofadenyl-oxyluciferinattheenzymesurface.Itisahomogeneoushighsensitivityfireflyluciferasereportergeneassaykitwithahalf-lifeofabout3hoursforthequantificationoffireflyluciferaseexpressioninmammaliancells,allowingbatchprocessinginmicroplates.

Glow-typeluciferaseassayslikeSteady-Luc™havelowerluminescencesignalcomparedtoflash-typeassays.Thesensitivityandlimitofdetectionoftheassaywilldependonluciferaseexpressionlevelsinyourexperimentalsystemaswellasluminometersensitivity.

BiotiumalsooffersSteady-Luc™FireflyHTSAssayKit(Lyophilized)(catalogno.30028L),anewpackagingformatthatincludeslyophilizedassaybufferforconvenientroomtemperatureshippingandstorageat-20°C.

KitComponents:

  • D-Luciferin
  • Steady-LucFireflyAssayBuffer

Features:

  • Simple:singlestephomogeneousassay.
  • Glowkinetics:luminescencehalflifeofabout3hours.
  • Economic:saveupto45%comparedtootherbrands.

MoreLuciferaseKits

CatalognumberProductDescription
30085FireflyLuciferaseAssayKit2.0Flash-typefireflyluciferaseassaykit.
30075FireflyLuciferaseAssayKit(Lyophilized)Fireflyluciferaseassaykitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C.
999235XFireflyLysisBufferExtralysisbufferforusewithFireflyLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well.
30028Steady-Luc™FireflyHTSAssayKitGlow-typefireflyluciferaseassaywithsignalhalf-lifeofabout3hoursforhigh-throughputscreeninginmulti-wellplates.
30028LSteady-LucFireflyHTSAssayKit(Lyophilized)Steady-Luckitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C.
30082RenillaLuciferaseAssayKit2.0Flash-typeRenillaluciferaseassaykit.
999125XPassiveLysisBufferExtralysisbufferforusewithRenillaLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well.
30081FireflyandRenillaLuciferaseSingleTubeAssayKitAssaykitformeasuringfireflyandRenillaactivitiessequentiallyinthesamesampleinasingletube.
99821-100mL1XPassiveLysisBuffer2.0ExtralysisbufferforusewithFirefly&RenillaSingleTubeAssayKits,onlyrequiredifusingwellsizeslargerthan24-well.
30020ATP-Glo™BioluminometricCellViABIlityAssayKitFlash-typeluminescence-basedassayformeasuringcellviabilityorATPconcentration.
22003MiniCellScrapersMini-cellscrapers,usefulforlysingcellsin96-well,48-well,or24-wellplates.

References

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