Fireflyluciferaseiswidelyusedasareporterforstudyinggeneregulationandfunction,andforpharmaceuticalscreening.Itisaverysensitivegeneticreporterduetothelackofendogenousactivityinmammaliancellsortissues.However,thelightproductionresultingfromthereactionleadstoformationofsuicidaladenyl-oxyluciferinattheenzymesurface.Itresultsinveryshorthalf-lifeofthelightemissionwithaflash-typekinetics.Becauseofthis,flash-typeluminescencemustbedetectedimmediatelyafterinitiatingthereaction,whichisnotcompatIBLewithbatchanalysisofsamples.
Biotium’sSteady-Luc™HTSassaysystemisaproprietarymixtureofsubstancesthatmodifytheenzymaticreactiontoproducealonglastingsignal(steadyglow)bypreventingtheformationofadenyl-oxyluciferinattheenzymesurface.Itisahomogeneoushighsensitivityfireflyluciferasereportergeneassaykitwithahalf-lifeofabout3hoursforthequantificationoffireflyluciferaseexpressioninmammaliancells,allowingbatchprocessinginmicroplates.
Glow-typeluciferaseassayslikeSteady-Luc™havelowerluminescencesignalcomparedtoflash-typeassays.Thesensitivityandlimitofdetectionoftheassaywilldependonluciferaseexpressionlevelsinyourexperimentalsystemaswellasluminometersensitivity.
BiotiumalsooffersSteady-Luc™FireflyHTSAssayKit(Lyophilized)(catalogno.30028L),anewpackagingformatthatincludeslyophilizedassaybufferforconvenientroomtemperatureshippingandstorageat-20°C.
KitComponents:
- D-Luciferin
- Steady-LucFireflyAssayBuffer
Features:
- Simple:singlestephomogeneousassay.
- Glowkinetics:luminescencehalflifeofabout3hours.
- Economic:saveupto45%comparedtootherbrands.
MoreLuciferaseKits
Catalognumber | Product | Description |
---|---|---|
30085 | FireflyLuciferaseAssayKit2.0 | Flash-typefireflyluciferaseassaykit. |
30075 | FireflyLuciferaseAssayKit(Lyophilized) | Fireflyluciferaseassaykitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C. |
99923 | 5XFireflyLysisBuffer | ExtralysisbufferforusewithFireflyLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well. |
30028 | Steady-LucFireflyHTSAssayKit | Glow-typefireflyluciferaseassaywithsignalhalf-lifeofabout3hoursforhigh-throughputscreeninginmulti-wellplates. |
30028L | Steady-LucFireflyHTSAssayKit(Lyophilized) | Steady-Luckitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C. |
30082 | RenillaLuciferaseAssayKit2.0 | Flash-typeRenillaluciferaseassaykit. |
99912 | 5XPassiveLysisBuffer | ExtralysisbufferforusewithRenillaLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well. |
30081 | FireflyandRenillaLuciferaseSingleTubeAssayKit | AssaykitformeasuringfireflyandRenillaactivitiessequentiallyinthesamesampleinasingletube. |
99821-100mL | 1XPassiveLysisBuffer2.0 | ExtralysisbufferforusewithFirefly&RenillaSingleTubeAssayKits,onlyrequiredifusingwellsizeslargerthan24-well. |
30020 | ATP-GloBioluminometricCellViABIlityAssayKit | Flash-typeluminescence-basedassayformeasuringcellviabilityorATPconcentration. |
22003 | MiniCellScrapers | Mini-cellscrapers,usefulforlysingcellsin96-well,48-well,or24-wellplates. |
References
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