NucView™405Caspase-3Substrate,1mMinDMSO,providesaconvenienttoolfordetectingapoptosisinintactcellsbasedoncaspase-3/7activityusingeitherconfocalmicroscopyorflowcytometry.

NucView™405Caspase-3Substrate,1mMinDMSO,providesaconvenienttoolfordetectingapoptosisinintactcellsbasedoncaspase-3/7activityusingeitherconfocalmicroscopyorflowcytometry.

Incontrasttootherfluorogeniccaspasesubstratesorfluorescentcaspaseinhibitorbased(FLICA)assays,NucView™caspase-3substratescanbeusedtodetectcaspase-3/7activitywithinindividualintactcellswithoutinhibitingapoptosisprogression.NucView™substratesconsistofafluorogenicDNAdyecoupledtothecaspase-3/7DEVDrecognitionsequence.Thesubstrate,whichisinitiallynon-fluorescent,penetratestheplasmamembraneandentersthecytoplasm.Inapoptoticcells,caspase-3/7cleavesthesubstrate,releasingthehigh-affinityDNAdye,whichmigratestothecellnucleusandstainsDNAwithfluorescence.Thus,NucViewcaspase-3substratesarebifunctional,allowingdetectionofcaspase-3/7activityandvisualizationofmorphologicalchangesinthenucleusduringapoptosis.Thestainingisalsoformaldehyde-fixable.

NucView™405 dyeisexcitedbythe405nmlaserlinefordetectionbyconfocalmicroscopyintheDAPIchannel,orbyflowcytometryinthePacificBlue®channel.NucView™405isidealforcaspase-3detectioninmulti-colorapplicationsforresearcherswhowishtoreservethegreenfluorescencechannelforotherdetectionreagents.

Note:NucView™405signalmaybedifficulttoobserveusingepifluorescencemicroscopy.Confocalmicroscopyusing405nmlaserexcitationisrecommendedforimaging.

NucView™405isalsoavailableat1mMinPBS(catalogno.10407),foruseinDMSO-sensitivecells.Biotiumalsooffersgreen NucView™488caspase-3substrateandkitsaswellasorangefluorescentNucView™530.

您买的GelRed是真的吗？ 我们都知道EB很毒，但是您知道AO（吖啶橙）比EB更毒吗？ AO（吖啶橙）也是能透过细胞膜完全嵌入细胞核DNA的。 正品GelRed的分子量在400以上，现在市面上大部分的假货、山寨货通过质谱分析分子量在200多，可以确定含有AO（EB的分子量在80几，AO的分子量是200以上） 为了您的身体健康，请从正规渠道购买正品，认准二维码防伪标，扫一扫，查真伪