AnnexinVfluorescentconjugatescanbeusedtodetectapoptoticcells.ThehumananticoagulantAnnexinVisa35-36kilodalton, calcium-dependentphospholipid-bindingproteinwithhighaffinityforphosphatidylserine(PS).Innormalviablecells,PSislocatedontheinnerleafletofthecytoplasmicmembrane.Inapoptoticcells,however,PSistranslocatedfromtheinnertotheouterleafletoftheplasmamembrane,whereitcanbedetectedbyfluorescentlylabeledAnnexinV.
It’savailablewiththefollowingdyes: CF™350, CF™405M,CF™488A,CF™555,CF™568,CF™594,CF™633,CF™640R,CF™647,CF™660R,CF™680R,FITC,Sulforhodamine101(TexasRed®),Biotin,R-PE,andAPC.
AlsoseeourNear-IRCF™DyeAnnexinVConjugates,suppliedlyophilizedandpreservative-free.
WealsoofferAnnexinVinthefollowingapoptosisassaykits:
- AnnexinV+NucView488
- AnnexinV+EthD-III
- AnnexinV+EthD-III+Hoechst
TexasRedisaregisteredtrademarkofThermoFisherScientific.
References
1.JImmunolMethods184,39(1995).
2.Blood84,1415(1994).
3.Cytometry43,134(2001).
4.FEBSLett.203,99(1986).
5.Biosci.Rep.7,289(1987).
DownloadalistofCFdyereferences.
