LysoView™dyeslabellysosomesinlivecells,andareavailablein3dyecolors:visibleredLysoView™540,far-redLysoView™633,andfar-redLysoView™650.
LysoView™540isaredfluorogenicdyewithpH-dependentfluorescenceforstainingacidicorganellesinlivecells.LysoView™540belongstoaclassoflysosomotropicdyesthatpossessweaklybasicaminegroups.ItishypothesizedthatprotonationofweakbasesunderacidicconditionscausesthedyestobetrappedinthelowpHcompartmentofthelysosome.Inaddition,thefluorescenceofLysoView™540ispH-sensitive,resultinginhighlyspecificlysosomalstainingwithouttheneedforawashstep.Thedyehasexcitation/emissionat541/634nmforimagingintheCy®3channel.
LysoView™633isafar-redfluorescentdyethatishighlyselectiveforlabelingandtrackingacidicorganellesinlivecells. ComparedtoLysoTracker®DeepRed,LysoView™633offershighlyspecificlysosomalstainingwithnowashsteprequired.Withabsorptionandemissionat634/659nm,thedyecanbedetectedusingaCy®5filterset.
LysoView™650isafar-redfluorescentlysosomestainwith absorption/emissionat650/675nm,whichiscompatiblewithsuper-resolutionimagingbySIMandSTED.
LysoView™540andLysoView™650aresuppliedas1000XstocksolutionsinDMSO.LysoView™633issuppliedasa lyophilizedsolid;afterreconstitutioninwater,eachvialyields100uLof1000Xdyestocksolution.
AlsoseeouruniqueUV-activatable Light-OnLysoView™555,whichisnon-fluorescentuntilitisactivatedbyUVlight.
CyDyeisaregisteredtrademarkofGEHealthcare.
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