Light-OnLysoView™555isaredfluorogeniclysosomedyewithpH-dependentfluorescence.Light-OnLysoView™dyeisuniqueamongcommerciallyavailablelysosomotropicdyesinthatitsfluorescenceincellsisactivatedbyexposuretoUVexcitation.

Light-OnLysoView™555isaredfluorogeniclysosomedyewithpH-dependentfluorescence.Light-OnLysoView™dyeisuniqueamongcommerciallyavailablelysosomotropicdyesinthatitsfluorescenceincellsisactivatedbyexposuretoUVexcitation.Insolution,thedyeshowspH-dependentfluorescencethatdoesnotrequireUVactivation.Incells,thedyeinitiallyshowslowfluorescence,butbriefexposuretoUVexcitationfromamercuryarclampthroughaDAPIfilteractivatesbrightredfluorescencelocalizingtolysosomes.

Wehypothesizethatthedyeassumesanon-fluorescentstructurethatcanbeswitchedtoafluorescentstructurebyUVexcitation.Mercuryarclampexcitationofthedyeusingotherfiltersets(FITC,rhodamine)ordyeexcitationwitha405nmlaserdoesnotactivatefluorescence.LysosomalfluorescencefadesseveralminutesafterUVexposure,butcanbere-activatedinthesamecellsmultipletimesbyexposuretoUVlight.ThereforethedyeprovidesanoveltoolforUV-activated,reversIBLefluorescenceimagingoflysosomes.Seetheproductprotocolunderthedownloadstabformoreinformation.

AlsoseeourredfluorescentLysoView™540,andfar-redfluorescentLysoView™633lysosomestains,whichdonotrequireUVactivation.

您买的GelRed是真的吗？ 我们都知道EB很毒，但是您知道AO（吖啶橙）比EB更毒吗？ AO（吖啶橙）也是能透过细胞膜完全嵌入细胞核DNA的。 正品GelRed的分子量在400以上，现在市面上大部分的假货、山寨货通过质谱分析分子量在200多，可以确定含有AO（EB的分子量在80几，AO的分子量是200以上） 为了您的身体健康，请从正规渠道购买正品，认准二维码防伪标，扫一扫，查真伪