ViaFluor™LiveCellMicrotubuleStainsarecell-permeabletaxolprobesforimagingthemicrotubulecytoskeletoninlivecells. Theyaresimple,rapidandsensitivestainsthatcanbeimagedwithoutawashstep.ViaFluor™488MicrotubuleStainhasgreenfluorescence(Ex/Em500/515nm)forimagingintheFITCchannel.ViaFluor™647MicrotubuleStainhasfar-redfluorescence(Ex/Em~650/~675nm) forimagingintheCy®5channel,andiscompatIBLewithsuper-resolutionimagingbySIMorSTED.
TaxolbindstopolymerizedtubulinandstABIlizesmicrotubules,resultingininhibitionofinhibitionofmitosis.However,fluorescenttaxolcompoundslikeViaFluor™647arelessdisruptiveofmicrotubuledynamicsandcelldivision,presumablyduetolowerbindingaffinityofthefluorescentprobecomparedtotaxolitself.ViaFluor™647doesnotshowcytotoxicitywithupto24hoursofexposureinimmortalizedcelltypes. ViaFluor™LiveCellMicrotubuleStains cannotbefixedafterstaining,andcannotbeusedwithfixedcellsortissues.
ViaFluor™MicrotubuleStainsaresuppliedasa1000XstocksolutionsinDMSO.Theycomewithavialof100mMverapamil,aneffluxpumpinhibitorthatmayimproveproberetentionandstainingincertaincelltypes.Notethatthisisahigherconcentrationthanpreviouslysuppliedwiththestains,toallowfortheuseofhigherverapamilconcentrations(10-100uM).Theoptimalconcentrationofverapamilmayvarybetweencelltypes.
Learnmoreaboutourotherfluorescentcellstains.
[/one_half_last]Cy5isaregisteredtrademarkofGEHealthcare.
您买的GelRed是真的吗？ 我们都知道EB很毒，但是您知道AO（吖啶橙）比EB更毒吗？ AO（吖啶橙）也是能透过细胞膜完全嵌入细胞核DNA的。 正品GelRed的分子量在400以上，现在市面上大部分的假货、山寨货通过质谱分析分子量在200多，可以确定含有AO（EB的分子量在80几，AO的分子量是200以上） 为了您的身体健康，请从正规渠道购买正品，认准二维码防伪标，扫一扫，查真伪
