Biotium’sCellBrite™NIRCytoplasmicMembraneDyesareready-to-usedyedeliverysolutionsthatcanbeaddeddirectlytonormalculturemediatouniformlylabelcellsinsuspensionoradherentcultures.
Carbocyaninedyeslabelcytoplasmicmembranesandintracellularmembranestructuresefficientlyandpermanently(1).Theyhavebeenusedastracersincell–cellfusion(2,3),cellularadhesion(4,5),andmigration(6)applicationsduetotheirpropertiesoflowcytotoxicityandhighresistancetointercellulartransfer.However,thelipophilicnatureofthesedyesposeanobstacletouniformcellularlabeling.AlthoughstructurallyrelatedPKHdyeshavebeendevelopedandoptimizedforcelllabeling,theprocedurerequiresmultiplestepsandsubjectscellstoaniso-osmoticmannitolloADIngmedium(7,8). Avarietyofcarbocyaninedyeswithdifferentopticalpropertiesareavailable,allowingcellpopulationstobemarkedwithdistinctfluorescentcolorsforidentificationaftermixing.Doublelabelingcanidentifycellsthathavefusedorformedstableclusters.
CellBrite™NIRCytoplasmicMembraneDyesarenovelnear-infraredcarbocyaninedyesforlabelingthecytoplasmicmembranesoflivingcells.Duetotheirlongemissionwavelengths,near-infraredcellmembranestainscanbeusedtolabelcellsfornear-infraredsmallanimalimagingstudiesfornon-invasiveimagingofcellmigrationandcellhoming(9).Thedyeshavelong18-carbonhydrophobictailsandanadditionalwater-solublegroup.Theseuniquechemicalstructureelementsmakethedyeseasytodissolvewhileprovidinghighlystablecytoplasmicmembranestaining,unliketraditionalcarbocyaninedyeslikeDiI,DiO,andDiR,whichareoftendifficulttodissolveorpronetoprecipitationduringcellstaining.
CellBrite™NIRCytoplasmicMembraneDyesareavailableinthefollowingcolors:NIR680,NIR750,NIR770,andNIR790.
BiotiumoffersotherCellBrite™CytoplasmicMembraneStainsinavarietyofcolors.Formoreinformation,downloadtheproductinformationsheet,orseeFrequentlyAskedQuestionsaboutCellBrite™CytoplasmicMembraneStains.
ProductInformation
| CatalogNo. | MembraneDye | Abs/Em |
|---|---|---|
| 30070 | CellBrite™NIR680 | 683/724nm |
| 30077 | CellBrite™NIR750 | 748/780nm |
| 30078 | CellBrite™NIR770 | 767/806nm |
| 30079 | CellBrite™NIR790 | 786/820nm |
References
Cat#30079
1.ScienceAdvances(2017),doi:10.1126/sciadv.1700521
