CellBrite™CytoplasmicMembraneLabelingKitsaredesignedtolabelcellcytoplasmicmembraneswithfluorescentdyes,availableinblue,green,orange,andred.Thelabelingisstableandnontoxic,suitableforlong-termtrackingofcells. BycombiningmultipleCellBrite™CytoplasmicMembraneStains,onecanlabelmultiplecellpopulationswithdifferentcolorsforstudiesofcell-cellinteractions.
CellBrite™stainsarebasedonlipophiliccarbocyaninedyes:CellBrite™BlueisbasedonDiB,CellBrite™GreenisbasedonNeuro-DiO,CellBrite™OrangeisbasedonDiI,andCellBrite™RedisbasedonDiD.Carbocyaninedyeslabelcytoplasmicmembraneandintracellularmembranestructuresefficientlyandpermanently.Theyhavebeenusedastracersincell–cellfusion,cellularadhesion,andmigrationapplicationsduetotheirpropertiesoflowcytotoxicityandhighresistancetointercellulartransfer.
Biotium’sCellBrite™CytoplasmicMembraneDyesaredyedeliverysolutionsthatcanbeaddeddirectlytonormalculturemediatouniformlylabelsUSPendedoradherentcellsinculture.CellBrite™dyesallowcellpopulationstobemarkedindistinctivefluorescentcolorsforidentificationaftermixing.Doublelabelingcanidentifycellsthathavefusedorformedstableclusters.
WealsoofferCellBrite™NIRCytoplasmicMembraneDyeswithemissioninthefar-red/near-infraredregionforimagingbyeithermicroscopyornear-infraredimagingdevices.
Kitsinclude:
- CellBrite™ fluorescentmembranedyepre-dissolvedinanoptimizedstainingsolution
- LoADIngbuffer(CellBrite™Blueonly)
Formoreinformation,downloadtheproductinformationsheet,orseeFrequentlyAskedQuestionsaboutCellBrite™CytoplasmicMembraneStains.
ProductInformation
| CatalogNo. | MembraneDye | Abs/Em |
|---|---|---|
| 30024 | CellBrite™Blue | 366/441nm |
| 30021 | CellBrite™Green | 484/501nm |
| 30022 | CellBrite™Orange | 549/565nm |
| 30023 | CellBrite™Red | 644/665nm |
References
1.JCellBiol103,171(1986).
2.JCellBiol135,63(1996).
3.AnnalsofAnatomy(2014),http://dx.doi.org/10.1016/j.aanat.2014.05.038
4.VeterinaryandComparativeOncology(2016),doi:10.1111/vco.12236
