Reporter genes are commonly used in cell biology research. 

 

OZ Biosciences offers reporter mRNAs that can be used as controls to study transfection and expression in mammalian cells using a variety of assays. They mimic fully processed mature mRNA.

These mRNAs are produced by in vitro transcription and stabilized at the 5" end by modified nucleotides capping (Cap1). They contain a poly(A) tail at the 3" end and are optimized to yield improved stability and performance.

They are modified with 5-methoxyuridine (5moU replaces U) to reduce innate immune responses.

 

We also offer unmodified mRNAs -- #MRNA9 (Tomato), #MRNA15 (GFP), #MRNA16 (F-Luc).

We recommend using our transfection reagents RmesFect or RmesFect Stem in order to reach high transfection efficiency and optimum protein expression level.

mRNA transfection provides several advantages :

• No need of nuclear uptake - protein expression directly in cytoplasm

• Faster protein expression than DNA transfection

• No genomic integration

• Protein expression directly related to mRNA quantity

• Perfect for transfecting slowing or non-dividing cells

• Protein expression in a total promoter-independent manner

• Transient transfection: mRNA based expression of proteins sustains for a limited time

 

 

• GFP mRNA has been designed to produce high expression level of Green Fluorescent Protein. It is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm. 

• F-Luc mRNA has been designed to produce high expression level of FireFly Luciferase protein. It is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.
• Tomato mRNA has been designed to produce high expression level of Orange Fluorescent Protein. The detection can be done by fluorescent or confocal microscopy. The produced Tomato has an excitation maximum at 551-557 nm and emission maximum at 579-583 nm. Tomato expression level can also be monitored by fluorescence-activated cell sorter analysis (FACS).

• mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in Discosoma sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is photostable and resistant to photobleaching. 
• β-galactosidase (β-gal) mRNA, codes for a protein product of the bacterial LacZ gene. β-gal catalyzes the conversion of β-galactosides into monosaccharides. it is a common marker gene used to assess transfection efficiency. 

 

Size: 20 µg, 100µg and 1mg.

For bulk, please contact us: order@ozbiosciences.com

Storage: -80°C

mRNAs ARE SHIPPED ON DRY ICE

Dry ice fee included in shipping cost

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The following items are 5 moU modified mRNAs -- if you need unmodified mRNAs, please visit this page.

OZ Biosciences的VaxOZ佐剂非常适合作为金标准参照物和任何免疫实验。也可以使用您自己的免疫刺激剂来定制它们。当前，佐剂仍然是提高常规和下一代疫苗效率的最重要策略。遗传佐剂是与特定抗原结合使用的免疫学试剂，可增强免疫反应。直接后果是与仅使用抗原相比，会产生更多的抗体和更长的免疫力，从而使疫苗所需的抗原剂量降至最低。特别是，基于DOTAP（1,2-油酰基--3-三甲基铵-丙烷）的阳离子脂质可诱导树突状细胞成熟，然后运输至引流淋巴结以激活抗原特异性T细胞。疫苗佐剂OZ生物科学VaxOZ佐剂是体内制造和质量控制的产品，仅用于研究目的。- AlumVax氢氧化物 / 磷酸盐，两个古典铝基佐剂- IFAVax， 不完全弗氏佐剂- CFAVax，完全弗氏佐剂- SqualVax，一个纳米油包水乳液-  CaLiVax-DOTAP， 对于脂质体介导的DNA或蛋白质疫苗基于阳离子脂质的组合物。