Overview

The E.Z.N.A.® Soil DNA Kit is formulated to isolate high purity cellular DNA from soil samples typically containing humic acid and other inhibitors of PCR. This kit uses a novel and proprietary method to isolate genomic DNA from a variety of environmental samples without organic extractions.

This kit has been successfully used to isolate DNA from Gram-positive and -negative bacteria, fungi, yeast, and algae that inhabit a range of samples including clay, sandy, peaty, chalky, or loamy soil samples. Isolated DNA can be used for most downstream applications, including PCR, Southern blot, and NGS analysis.

• Reliable – Reproducible DNA purification from a variety of sample sources
• High quality – Ready-to-use DNA eliminating PCR inhibitors using proprietary inhibitor removal technology
• Yield – Efficient purification of DNA from even specialized samples
• Ease of use – Contains glass beads pre-filled in 2 mL vials

Protocol and Resources

Product Documentation & Literature

Product Data

DNA purified from soil samples using E.Z.N.A.® Soil DNA Kit has higher and more consistent yield than using a leading competing product.

Figure 1. Comparison of DNA extraction method from soil samples. DNA yield determined with fluorescence-based dye quantification. 50 µL ZymoBIOMICS™ Microbial Community Standard was added to 200 mg soil samples and DNA was extracted using manufacturer’s recommended protocols. DNA was eluted in 100 µL for both manufacturers.

DNA purified from soil samples using E.Z.N.A.® Soil DNA Kit has better PCR performance than using a leading competing product.

Figure 2. Comparison of Ct values. 20 µL SYBR Green qPCR reaction. 50 µL ZymoBIOMICS™ Microbial Community Standard was added to 200 mg soil samples and DNA was extracted using manufacturer’s recommended protocols. DNA was eluted in 100 µL for both manufacturers.

E.Z.N.A.® Soil DNA Kit performs especially better for gram-positive bacteria than a leading competing product.

Figure 3. DNA yield by bacterial classes. DNA yield determined with fluorescence-based dye quantification. 0.5 mL cultured Gram-positive and Gram-negative bacteria were added to corresponding 200 mg soil samples and DNA was extracted using manufacturer’s recommended protocols. DNA was eluted in 100 µL for both manufacturers.

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