pLV-[hsa-mir-941-1_941-2_942-3_942-4]plasmid
HumanmicroRNA(hsa-mir)precursorsandapproximately100-bpupstreamanddownstreamflankinggenomicsequenceswerePCRamplifiedandclonedintoaself-inactivated(SIN)lentiviralvectortogeneratepLV-miRNAvectors.Thecloningsiteofpre-miRNAgenomicfragmentsiswithintheintronofhumanEF1apromoterregion.ThemiRNAofinterestcanbedeliveredintocellsbytransienttransfectionofthepLV-miRNAplasmidorlentiviraltransductionwhilethemiRNAlentiviralstockispreparedfromcotransfectingHEK293TcellswiththepLV-miRNAplasmidandlentiviralpackagingvectormix.
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Cat#:mir-pc13
Biosettia专注于开发和商业化独特的技术,为生命科学研究提供更好的解决方案。该公司致力于提供创新的工具和特殊试剂,以协助全基因组功能分析和诱导多能干细胞(IPSC)的产生。
Biosettia is a San Diego-based company that provides molecular biology products and services to support researchers worldwide. Using our highly efficient lentiviral system, we have assisted many academic institutes, biotech and pharmaceutical companies in doing gene expression & inhibition-related studies.
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