品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
pLV-[hsa-mir-15a_16-1]plasmid
HumanmicroRNA(hsa-mir)precursorsandapproximately100-bpupstreamanddownstreamflankinggenomicsequenceswerePCRamplifiedandclonedintoaself-inactivated(SIN)lentiviralvectortogeneratepLV-miRNAvectors.Thecloningsiteofpre-miRNAgenomicfragmentsiswithintheintronofhumanEF1apromoterregion.ThemiRNAofinterestcanbedeliveredintocellsbytransienttransfectionofthepLV-miRNAplasmidorlentiviraltransductionwhilethemiRNAlentiviralstockispreparedfromcotransfectingHEK293TcellswiththepLV-miRNAplasmidandlentiviralpackagingvectormix.
| PlasmidDescription | Advantages | DownloadGenbank | Protocols | PurchaseControl |


