Hito Van Gieson OptimStain™ Kit is designed based on the Van Gieson and Weigert iron hematoxylin staining method with improved and simplified procedures. This kit can be used for simultaneously demonstrating the morphological details of collagen fibers.Hito Van Gieson OptimStain™ Kit is made in a ready-to-use format and provides high quality, reliable and sensitive staining of collagen fibers.Hito Van Gieson OptimStain™ Kit has been tested extensively on the tissues from several species of animals and it is a simple solution for your research.

Kit Contents
Solution-1A	125 ml
Solution-1B	125 ml
Solution-2 (HITO VAN GIESON STAIN)	250 ml
Staining jars	4
User Manual and MSDS	1
Before using Hito Van Gieson OptimStain™ Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit): Cryostat or Microtome, Light microscopeParaffin embedding equipment (or paraffin sections)Hito Bouin’s Plus Solution (Cat# HTSHS0104, for paraffin sections preparation)Dry ice, isopentane, O.C.T. compound (for frozen sections), 4% PFA (Cat# HTSHS0102), ethanol, xylene, double distilled or deionized waterSlides, coverslipsStaining jars for slides washResinous mounting medium  Hito Van Gieson OptimStain™ Kit Manual and MSDS