PP2C Source: Bovine brain
Purity: > 90% by SDS-PAGE, apparent Mr ~ 45-kDa
Supplied: 1 μg in 50 μl 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA and 50% glycerol.
Activity: ~ 1000 units/mg with casein as substrate. One unit is the amount of PP2C that releases 1 nmol of inorganic phosphate from 32P-labeled casein per min. Maintain preparations in aliquots at -70° C. Avoid repeated thawing.
Synonyms: Protein Phosphatase 2C
GloboZymes PP2C preparations are useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and to evaluate the effects of test substances on the activity of the phosphatase.