I2PP2A(β)
Source: Recombinant human kidney produced in E. coli
Purity: > 90% by SDS-PAGE, apparent Mr ~ 41-kDa.
Supplied: 1 μg in 50 μl 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA, 0.1% Brij-35 and 10% glycerol. Maintain preparations in aliquots at -70° C. Avoid repeated thawing.
Synonyms: Taf-1a; Protein Phosphatase 2A Inhibitor Protein 2β; I2βPP2A
Background: I2PP2A(β) inhibits protein phosphatase 2A (PP2A) activity potently with ki ~ 0.1 – 0.5 nM with Myelin Basic Protein (MBP), Histone H1 and several other substrates but not with Casein. It stimulates PP1 in the presence of Mn2+, and regulates histone binding to DNA, transcriptional activity and chromatin condensation.
References: Li M, Makkinje A, Damuni Z. (1996) J Biol Chem 271, 11059-11062; Nagata, K. et al (1995) Proc Natl Acad Sci U S A 92, 4279-4283
