- Each preadipocyes lot functionally characterized
- Same quality standard as the PrimaPure human cell products
- Each lot tested negative for HIV, Hepatitis B & C, mycoplasma, bacteria, yeast, and fungi
- Performance guaranteed with Genlantis media and reagents
Human Preadipocytes (HPAd) are fibroblast like precursor cells derived from human adipose tissue. They are cryopreserved at the end of primary culture and can be propagated two passages prior to differentiating into Human Adipocytes (HAd). HPAd derived HAd were used to study insulin-stimulated glucose transport, growth hormone enhanced lypolysis, and obese gene expression. Significant differences in lipolysis and leptin production are observed in HPAd and HAd from human omental and subcutaneous adipose tissue.
Oil Red O stained lipid accumulation
Genlantis provides HPAd from subcutaneous adipose tissue at various sites and adipose depot on heart. All HPAd are characterized positive for oil droplet accumulation.
Cell Type | Description | Quantity | Cell Passages | Recommended Media | Cat. No. | |
HPAd (Heart, Adult) | Human preadipocytes | >500,000 cells | 1st | Human Preadipocyte Growth Medium | PH80205A(cells only) | |
PH80205AK(complete system*) | ||||||
HPAd (Subcutaneous, Adult) | Human preadipocytes | >500,000 cells | 1st | Human Preadipocytel Growth Medium | PH80305A(cells only) | |
PH80305AK(complete system*) | ||||||
* Each Complete System contains an ampoule of cryopreserved cells (> 500,000 cells), 500 ml growth medium, and a Subculture Reagent Kit (including 100 ml each of HEPES Buffered Saline Solution, trypsin/EDTA, and trypsin neutralizing solution). |
StoragePreadipocytes are cryopreserved immediately after purification and delivered on dry ice. Place cryovials immediately in liquid nitrogen upon arrival. Store the growth medium at 4°C in the dark. Store the subculture reagent at -20°C upon receipt and at 4°C upon thawing.