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As a T-cell independent carrier (TI carrier), coupling Ficoll with haptens has been shown to produce robust antigen-specific primary immune memory1. Our method for amino functionalization yields stable primary amine groups. We carefully control the amine:polymer ratio and process to remove excess amine reagent. Custom Ficoll preparations are available upon request. GE Healthcare’s trademark Ficoll is a highly branched, 400kDa, hydrophilic polysaccharide widely used for blood separation.
1. Inman, JK, Thymus-independent antigens: The preparation of covalent, hapten-Ficoll conjugates. J Imm. 114:705, 1975.
