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ESIBIO/ESI-035 Human Embryonic Stem Cell Line/≥ 5 x 10e5 cells/ES-701

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¥19000.00
货号:ES-701
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品牌:ESIBIO
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Morphology of ESI-035 at passage 18 in feeder-free culture.Zoom

  • Morphology of ESI-035 at passage 18 in feeder-free culture.
  • Karyogram of ESI-035 at passage 18 displays a normal karyotype (46, XX).
  • STR profile of ESI-035.

ESI-035 Human Embryonic Stem Cell Line

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Research grade hESC line derived from cGMP compliant banks grown in feeder-free culture conditions.
SKU: ES-701
$950.00

Order Offline:

T: 877.636.4978 E: orders@esibio.com

F: 510.648.3255

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Description

Details

ESI BIO offers the highest quality human embryonic stem cell (hESC) lines that are among the best-characterized and documented lines commercially available today. The ESI cell lines are NIH registered and backed with donor history and testing information in best compliance with current Good Tissue Practice (cGTP) and conform to Global Ethical Standards and Clinical Cell Regulations. ESI hESCs were derived under current Good Manufacturing Practice (cGMP) conditions on human fibroblast feeder layers and are available in both research and clinical-grade formats. For most investigators, research banks of the ESI hESC lines provide a convenient entry point, while GMP compliant banks of these same cells create seamless translation into clinically relevant studies.The ESI hESCs are well characterized in regards to genomic integrity, pluripotency status, and culturing behavior. Each line has been karyotyped, STR fingerprinted, has high viability thawing recovery, is characterized by pluripotency marker expression, and is free of mycoplasma, pathogens, bacteria, yeast, and fungus. In addition to the standard pluripotency and karyotype analysis, the complete genome sequence is available for purchase.
NIH registered lines

Details

Details

FormatResearch grade
Culture ConditionsFeeder independent in defined medium
Size≥ 5 x 10e5 cells
Passage Number<>
StorageLiquid nitrogen
Expanded and Banked byBioTime, Inc.
Karyotype46, XX
Positive Stem Cell MarkersOct4, Sox2, TRA-1-60, TRA-1-81
Technical Documents

ESI-035 Technical Data Sheet

Product Support

EDTA-based Passaging of ESI Human Embryonic Stem Cells Protocol

Brochures

Product Information Sheet

ReferencesNIH Human Embryonic Stem Cell Registry [Number 0129] »Crook, J.M., et al. (2007) The generation of six clinical-grade human embryonic stem cell lines. Cell Stem Cell 1: 490-494. PMID: 18938745.Funk, W.D., et al. (2012) Evaluating the genomic and sequence integrity of human ES cell lines; comparison to normal genomes. Stem Cell Res 8(2): 154-164. PMID: 22265736.
cGMP Cell Line AvailabilityRequest more information »

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ESIBIO的TGF-βI型受体ALK5激酶,I型激活素/淋巴结受体ALK4和I型淋巴结受体ALK7的选择性抑制剂(IC 50值分别为12、45 和7.5 nM)。A83-01阻断Smad2的磷酸化并抑制TGF-β诱导的上皮-间充质转化。仅弱抑制ALK-1,-2,-3,-6和MAPK活性。A83-01已显示抑制大鼠iPSC分化并增加克隆扩增效率。它有助于维持人iPSC的均质性和长期体外自我更新。