Raf1 (Phospho-Ser259) AntibodyPDF
Catalog Number:
E011006-2Amount:
100μg/100μlForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Raf-1 around the phosphorylation site of serine 259 (S-T-SP-T-P).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
Raf-1 (phospho-Ser259) antibody detects endogenous levels of Raf-1 only when phosphorylated at serine 259.Reactivity:
Human, Mouse, RatApplications:
WB: 1:500~1:1000 IHC: 1:50~1:100Swiss-Prot No.:
P04049References:
Dougherty M K, et al. (2005) Mol Cell. 17(2): 215-224.Hekman M, et al. (2005) FEBS Lett. 579(2): 464-468.Avruch J,et al. (1994) Trends Biochem. Sci. 19, 279-283.Chong H, et al. (2001) EMBO J. 20, 3716-3727.King A J, et al. (1998) Nature. 396:180-183.Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf-1 (phospho-Ser259) antibody (E011006).
Western blot analysis using Raf-1 (Ab-259) antibody (E021006, Line 1, 2, and 3) and Raf-1 (phospho- Ser259) antibody (E011006, Line 4, 5, and 6).
Price:
225$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells