β-tubulin Mouse Monoclonal Antibody(Zebrafish Specific)PDF
Catalog Number:
E12-142Amount:
100μg/100μlBackground:
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.Form of Antibody:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 year. Do not aliquot the antibody.Specificity/Sensitivity:
Theβ-tubulin antibody can detects Zebrafish endogenousβ-tubulin protein.Reactivity:
ZebrafishApplications:
WB: 1:5,000-20,000 IHC: 1:200 IF: 1:200Western blot analysis of Zebrafish skeletal muscle with β-tubulin Mouse mAb(Zebrafish Specific) diluted at 1:5,000.
Price:
150$