Diatheva蚂蚁淘在线

Description

Listeria monocytogenes is a facultative intracellular bacterial pathogen that escapes from a host vacuolar compartment and grows rapidly in the cytosol. Listeriolysin O (LLO), one of its major virulence factors, is a secreted pore-forming protein essential for the escape of L. monocytogenes from the vacuole formed upon initial internalization.In addition, LLO influences the levels of intracellular calcium and stimulates phosphoinositide metabolism to favour bacterial entry. It is also a potent aggregator of lipid rafts and suppresses the host immune response. Phagosomal membrane disruption is enhanced by the bacterial phosphatidylinositol-specific and broad-range phospholipases C, PI-PLC (plcA) and PC-PLC (plcB), respectively. Enzymes cooperate with LLO in the lysis of primary and secondary vacuoles.

Additional information

Size	200 µL
Application	Clinical diagnostics\|Life Sciences
Product type	Reagents for research
Reagent type	Antibody
Category	Lysteria monocytogenes
Subcategory	Polyclonal anti LLO antibody
Antibody type	Primary antibody
Immunogen	Recombinant purified Listeriolysin O proteinexpressed in E.coli

Technical information

Accession number	GenBank AAP33758.1
Size	200 µL
Purity	Purified by Protein A affinity Chromatography
Form	Liquid. Supplied in 100mM sodium citrate, 50mM Tris and 0.05% v/v glycerol. Neutral pH.
Shipping/storage conditions	Shipped at 2-8°C. When stored at +4°C the antibody is stable for 18 months. For extended storage up to 24 months, the solution may be frozen at -20°C in working aliquots. Avoid repested freezing and thawing cycles.
Usage	For Laboratory Research Use Only
Source	Rabbit
Specificity	Listeriolysin O from infected specimens
React with	Listeryolisin Ofrom L. monocytogenes
Recommended dilutions	Recommended starting dilutions can vary lot-to-lot. Consult the suggested starting dilutions on the top of the page for lot specific values.

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Datasheet

References

A Novel Role of Listeria monocytogenes Membrane Vesicles in Inhibition of Autophagy and Cell Death	PUBMED ID: 28516064
Cold-Shock Domain Family Proteins (Csps) Are Involved in Regulation of Virulence, Cellular Aggregation, and Flagella-Based Motility in Listeria monocytogenes	PUBMED ID: 29124040

Related products

Rabbit Anti Listeriolysin O affinity purified Cholesterol-dependent cytolysins (CDCs) are produced by a large number of pathogenic gram–positive bacteria. A member of this family, listeriolysin O (LLO), is produced by the intracellular pathogen Listeria monocytogenes. Polyclonal anti Listeriolysin antibody affinity purified is designed for the specific detection of LLO in in infected speciment as an useful tool in the research of Listeria monocytogenes infections.The affinity purification protocol garantees an improvement on the specificsignal	ANT0016
Recombinant Listeriolysin-O (LLO) Recombinant Listeriolysin O (LLO) is a protein produced in E. coli. LLO is a single polypeptide protein composed of 529 residues lacking the secretion signal peptide (1-25 aa).	REP0007
Recombinant p24 BIOTIN Recombinant p24 is a 24 kDa, full-length protein produced in E. coli and conjugated with NHS Biotin	REP0024
Recombinant Listeriolysin-O (LLO) Recombinant Listeriolysin O (LLO) is a protein produced in E. coli. LLO is a single polypeptide protein composed of 529 residues lacking the secretion signal peptide (1-25 aa).	REP0006
Recombinant Listeriolysin-O PEST minus Recombinant Listeriolysin O without PEST sequence and with haemolytic activity is a protein produced in E. coli.LLO is a single polypeptide protein encoded by the hlyA gene and composed of 529 residues. PEST sequence is 19 amino acids peptide located at the protein NH 2-terminus.	REP0022

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Diatheva多病原体富集培养基500克在分离程序之前，可以使用多病原体富集培养基肉汤富集食品样品中的沙门氏菌，单核细胞增生李斯特菌和大肠杆菌O157。沙门氏菌，单核细胞增生李斯特氏菌和大肠杆菌O157被公认为是主要的食源性病原体，因为它们继续与诸如乳制品之类的高度受欢迎的食品相关联。最重要的是，这些病原体具有很高的流行率和死亡率，并参与了最近的几次暴发（Lynch等，2006）。多病原体富集培养基可提供营养物质和稳定的pH条件，以提高单核细胞增生李斯特菌沙门氏菌的回收率。和大肠杆菌O157细胞。