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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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商品描述
SpeciesReactivity | Multispecies(General) |
---|---|
Format | 96wellplate |
DetectionRange | 781.25-200000ng/mL |
Sensitivity | 271ng/mL |
SampleType | Serum,plasmaandotherBIOLOGicalfluids |
AssayTime | 2hours |
GeneName | Alpha-Tocopherol |
GeneAliases | VE;VitaminE;α-TCP;α-Tocopherol |
Application | CLIA |
AssayType | Competitive |
DetectionMethod | Chemiluminescent |
ShippingCondition | Blueice |
StorageInstructions | -20°C.Bringallreagentstoroomtemperaturebeforebeginningtest.Thekitmaybestoredat4°Cforimmediateusewithintwodaysuponarrival.Resealanyunusedstripswithdesiccantpack.Minimizefreeze/thawcycles. |
PrecautionOfUse | Donotallowtocontactskinoreyes.Calibrators,controlsandspecimensamplesshouldbeassayedinduplicate.Oncetheprocedurehasbeenstarted,allstepsshouldbecompletedwithoutinterruption. |
ExpiryDate | 8months |
Specificity | ThisassayhashighsensitivityandexcellentspecificityfordetectionofAlpha-Tocopherol(TCPa).Nosignificantcross-reactivityorinterferencebetweenAlpha-Tocopherol(TCPa)andanalogueswasobserved. |
TestPrinciple | Themicroplateprovidedinthiskithasbeenpre-coatedwithamonoclonalantibodyspecifictoAlpha-Tocopherol(TCPa).AcompetitiveinhibitionreactionislaunchedbetweenbiotinlabeledAlpha-Tocopherol(TCPa)andunlabeledAlpha-Tocopherol(TCPa)(Standardsorsamples)withthepre-coatedantibodyspecifictoAlpha-Tocopherol(TCPa).Afterincubationtheunboundconjugateiswashedoff.Next,avidinconjugatedtoHorserADIshPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.TheamountofboundHRPconjugateisreverseproportionaltotheconcentrationofAlpha-Tocopherol(TCPa)inthesample.ThenthemixtureofsubstrateAandBisaddedtogenerateglowlightemissionkinetics.Uponplatedevelopment,theintensityoftheemittedlightisreverseproportionaltotheAlpha-Tocopherol(TCPa)levelinthesampleorstandard. |
AssayPrecision | Intra-assayPrecision(Precisionwithinanassay):3sampleswithlow,middleandhighlevelAlpha-Tocopherol(TCPa)weretested20timesononeplate,respectively.Inter-assayPrecision(Precisionbetweenassays):3sampleswithlow,middleandhighle |
StABIlity | Thestabilityofkitisdeterminedbythelossrateofactivity.Thelossrateofthiskitislessthan5%withintheexpirationdateunderappropriatestoragecondition.Tominimizeextrainfluenceontheperformance,operationproceduresandlabconditions,especiallyroomtemperature,airhumidity,incubatortemperatureshouldbestrictlycontrolled.Itisalsostronglysuggestedthatthewholeassayisperformedbythesameoperatorfromthebeginningtotheend. |
Restrictions | Forresearchuseonly.Notfordiagnosticprocedures. |
QualitySystems | TheKitismanufacturedatISO9001andISO13485certifiedfacilities. |
ResearchArea | Metabolicpathway;Nutritionmetabolism; |