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Biomatik/Human Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit (CACNa1B) ELISA kit, Cat#EKU02864/96 Tests/EKU02864-96 Tests

价格
¥14700.00
货号:EKU02864-96Tests
浏览量:103
品牌:Biomatik
服务
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商品描述

Format

96-WellPlate

SpeciesReactivity

Homosapiens(Human)

SampleType

Tissuehomogenates,CelllysatesandotherBIOLOGicalfluids

DetectionRange

0.312-20ng/mL

Sensitivity

0.129ng/mL

EstimatedTurnaroundTime

7-11businessdays

TargetName

CalciumChannel,VoltageDependent,N-Type,Alpha1BSubunit

TargetSynonyms

CACNN,CACNL1A5,BIII,BraincalciumchannelIII,Calciumchannel,Ltype,alpha-1polypeptideisoform5,Voltage-gatedcalciumchannelsubunitalphaCav2.2

Precision

Intra-assayPrecision(Precisionwithinanassay):3sampleswithlow,middleandhighlevelCalciumChannel,VoltageDependent,N-Type,Alpha1BSubunitweretested20timesononeplate,respectively.Inter-assayPrecision(Precisionbetweenassays):3sampleswithlow,middleandhighlevelCalciumChannel,VoltageDependent,N-Type,Alpha1BSubunitweretestedon3differentplates,8replicatesineachplate.CV(%)=SD/meanX100;Intra-Assay:CV<10%;inter-assay:><>

DetectionMethod

Colorimetric

AssayTime

3hours

Application

AssayType

Double-antibodySandwich

ShippingCondition

Icepacks

Storage

Shortterm:4°C;Longterm:seemanual.

PrecautionofUse

TheStopSolutionisacidic.Donotallowtocontactskinoreyes.

ExpiryDate

8months

UniProtID

Q00975

Specificity

ThisassayhashighsensitivityandexcellentspecificityfordetectionofCalciumChannel,VoltageDependent,N-Type,Alpha1BSubunit

CrossActivity

Nosignificantcross-reactivityorinterferencebetweenCalciumChannel,VoltageDependent,N-Type,Alpha1BSubunitandanalogueswasobserved.

AssayProcedure

1.Prepareallreagents,samplesandstandards;2.Add100µLstandardorsampletoeachwell.Incubate2hoursat37°C;3.Aspirateandadd100µLpreparedDetectionReagentA.Incubate1hourat37°C;4.Aspirateandwash3times;5.Add100µLpreparedDetectionReagentB.Incubate30minutesat37°C;6.Aspirateandwash5times;7.Add90µLSubstrateSolution.Incubate10-20minutesat37°C;8.Add50µLStopSolution.Readat450nmimmediately.

StABIlity

Thestabilityofkitisdeterminedbythelossrateofactivity.Thelossrateofthiskitislessthan5%withintheexpirationdateunderappropriatestoragecondition.Tominimizeextrainfluenceontheperformance,operationproceduresandlabconditions,especiallyroomtemperature,airhumidity,incubatortemperatureshouldbestrictlycontrolled.Itisalsostronglysuggestedthatthewholeassayisperformedbythesameoperatorfromthebeginningtotheend.

Restrictions

ForResearchUseOnly.Notforuseinvitrodiagnosticprocedures,druguse,orforadmiNISTrationtohumansoranimals.

QualitySystems

ThekitismanufacturedatISO9001andISO13485certifiedfacilities.

QualityAssurance

BiomatikELISAkitsarenottypicallypre-assembledasfinishedproductsduetolimitedshelflife.Beforeshipping,eachkitisassembledandtestedtoensurethatitmeetsspecificationsbeforeshipping.MinorchangesmayoccurtotheDetectionRange,Sensitivity,andPrecision.AllkitsaretestedtoconfirmthattheyfallwithintheirdefinedInter-andIntra-assaycoefficientofvariation.PleasealwaysrefertothehardcopymanualincludedinThekitbeforeexperiment.

ResearchArea

Signaltransduction
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