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| Product Code | opr0009 |
|---|---|
| Constituents | Reagent Cov A Reagent Cov B |
| Lysat Type | "0 |
| Principle | Chemiluminescence is defined as the light emitted by a chemical reaction. Horse radish peroxidase (HRP), in the presence of hydrogen peroxide, catalyses the oxidation of a cyclic diacyl hydrazide, such as luminol, to an excited reaction product which on spontaneous change to a stable form emits light. A dramatic increase in the light output can be achieved by the presence of enhancers. |
|---|---|
| Applications | Western Blot, Dot Blot |
| Recommendations | Chemiluminescence is defined as the light emitted by a chemical reaction. Horse radish peroxydase (HRP), in the presence of hydrogen peroxide, catalyses the oxidation of a cyclic diacyl hydrazide, such as luminol, to an excited reaction product which on s |
| Storage | Stored at +4°C |
| Product type | Reagents |
| Preparation method | To be used extemporaneously or can be kept at +4°C for few hours : Add 1 drop of oxidant Cov B to 1 mL of substrat Cov A (125 µL of reagent covers 1 cm2 membrane). Detection : Immerse the membrane in Covalight reagent from 1 to 10 minutes. Shake of excess reagent and cover the membrane with a plastic sheet before to exposure. |
* Lassot I et al. Cell Rep. 2018 Nov 27:25(9):2484-2496.e9* LM Bloyet et al.Sci. Adv. 2019, 5 eaaw3702* Lassot I et al. Cell Rep. 2018 Nov 27:25(9):2484-2496* Chapuy-Regaud S et al. Biochimie. 2017 May 5.* Brosse A et al. Nucl. Acids Res. 2016 Jul 20.* Bloyet LM et al. J Virol. 2016 May 11.* Fischer K. TBX3-Mutationsanalysen und Konstruktion einer Zelllinie für TBX2-Inhibitorscreenings. Ph.D. Thesis 2015.* Julien M et al. Biochim Biophys Acta. 2015 Jun 14. pii: S0167-4889(15)00193-7.* Romanets-Korbut O et al. Cell Mol Immunol. 2015 Jun 15.* Couderc C. et al. Mol Cell Endocrinol. 2014 Sep 16. pii:S0303-7207(14)00284-6.* Schnorr S et al. Pain. 2014 Jun:155(6):1079-90.* Brot S, et al. Eur J Neurosci. 2014 Jul 12.* Yakoub S et al. J Cancer Sci Ther 2014, 6.4* Bollard J et al. Neuroendocrinology. 2013 Jan 23.* Perez Alea M et al. Amino Acids. 2013 Jan:44(1):245-50* Chauvelier-Grouiller S. Caractérisation épigénétique du lymphome du manteau. Ph.D. Thesis 2011
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- Three differents ECL Reagent were used with an exposure times of 2 min 30.1: Covalight® (opr0009)2: Covalight® Plus (opr1102) 3: Covalight® Xtra (opr1101)">
