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| Product Code | opr0027 |
|---|---|
| Uniprot ID | P21980 |
| Name | Transglutaminase-2 (TGase-2) |
| Appearance | White lyophilized solid |
|---|---|
| Constituents | The TG is lyophilised from 50mM NaH2PO4, 150 mM NaCl, pH8 and less than 0.1 mM Imidazole. Sample contains maltodextrin His6_rhTG2 is a Ca2+ dpt enzyme. |
| Storage | Store working aliquots at a minimum of -20°CAvoid repeated freezing and thawing |
| Description | His6-rhTG2 is based on the TGM2-allele from I.M.A.G.E.-clone IMAGp958L121020 isolated from neuroblastoma cells of the human brain. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MAHHHHHHAEELV….His6-rhTG2 is produced in E. coli and purified by ion metal chelating chromatography to more than 90 % purity. |
| Produced in | Escherichia coli |
| Species | Human |
| Form | Recombinant |
| Gene ID | 7052 |
| Labelling | His-Tag |
| Preparation method | The Transglutaminase is activated with 10 mM Ca2+; due to the precipitation of Calcium Phosphate a buffer exchange (e. g. Tris-Buffer) prior to activation is highly recommended. |
| PDB ID | 1FAU |
| Molecular weight | 78 kDa |
| Principle | His6-rhTG2 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG2 may also be used for immunoprecipitation. |
| Purity | > 90 % |
| Recommendations | Add the desired volume of H2O to the vial of lyophilized powder. |
| Product type | Proteins |
| Applications | Immunoprecipitation, Functional Assay |
| Research areas | Transglutaminase |
