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| Product Code | opr0036 |
|---|---|
| Uniprot ID | P21980 |
| Name | Transglutaminase-2, TGase-2 |
| Appearance | White lyophilized solid. |
|---|---|
| Constituents | 20 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM DTT, 1 mM EDTA, 10 mg/mL Sucrose |
| Storage | Store lyophilized product at -80°CAvoid repeated freezing and thawingDelivery at ambient temperature is possible. |
| Description | His6-rhTG2 is based on the TGM2-allele from I.M.A.G.E.-clone IMAGp958L121020 isolated from neuroblastoma cells of the human brain. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MAHHHHHHAEELV….His6-rhTG2 is produced in E. coli and purified by ion metal chelating chromatography to more than 90 % purity. |
| Produced in | Escherichia coli |
| Species | Human |
| Form | Recombinant |
| Gene ID | 7052 |
| Labelling | His-Tag |
| Preparation method | The Transglutaminase is activated with 10 mM Ca2+, due to the precipitation of Calcium Phosphate a buffer exchange (e. g. Tris-Buffer) prior to activation is highly recommended |
| PDB ID | 1FAU |
| Molecular weight | 78 kDa |
| Principle | His6-rhTG2 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines. It may also be used for immunoprecipitation. |
| Purity | > 95 % |
| Recommendations | Add distilled water (see specific datasheet for the exact volume). Rotate vial gently until solid dissolves. |
| Product type | Proteins |
| Applications | Immunoprecipitation, Functional Assay |
| Research areas | Transglutaminase |
