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Cell Technology/Fluoro Sarcosine/100/SARC100

价格
¥6200.00
货号:SARC100
浏览量:127
品牌:Cell Technology
服务
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商品描述
  • Description
  • Additional Information
  • Readable Documents
  • Assay Principle
  • Reviews

Introduction

Sarcosine is natural amino acid that is an important intermediate in the metabolism of choline. Sarcosine is an important component of proteins and plays a significant role in metabolic processes of living cells as a source of serine, creatine, purines and glutathione etc. Sarcosine is present in food sources like legumes, eggs, ham, turkey etc. It is used in a variety of industrial applications such as manufacturing of tooth paste and biodegradable surfactants. Sarcosine was recently reported to activate prostate cancer cells and as a possible marker for prostate cancer progression to metastasis(1). It is also used in adjunctive treatment for Schizophrenia (2) and depression (3).

Cell Technology’s Fluoro Sarcosine assay provides a reliable, sensitive fluorimetric method for the quantification of sarcosine in biological samples.The Fluoro Sarcosine detection kit utilizes a non-fluorescent detection reagent, which is reduced via an enzyme-coupled reaction in the presence of sarcosine. A sarcosine standard curve is generated to quantify sarcosine in the samples.

Key Benefits

  • Easy to Use – No Wash Assay.
  • Detection of Sarcosine in cells, serum, tissue extracts.
  • Easy to Use 96-well Fluorescent Plate reader readout.

Additional information

Kit Size

100

Document Title
Fluoro Sarcosine Protocol
Fluoro Sarcosine Datasheet
msds.fluoroSarcosine
Reference
Sreekumar, Arun; Poisson, Laila M.; Rajendiran, Thekkelnaycke M.; Khan, Amjad P.; Cao, Qi; Yu, Jindan; Laxman, Bharathi; Mehra, Rohit et al. (2009). "Metabolomic profiles delineate potential role for sarcosine in prostate cancer progression". Nature 457 (7231): 910. doi:10.1038/nature07762. PMID 19212411.
Lane H, Huang C, Wu P, Liu Y, Chang Y, Lin P, Chen P, Tsai G (2006). "Glycine transporter I inhibitor, N-methylglycine (sarcosine), added to clozapine for the treatment of schizophrenia". Biol Psychiatry 60 (6): 645–9. doi:10.1016/j.biopsych.2006.04.005. PMID 16780811.
images/CellTechnology/NCT00977353 Clinicaltrials.gov "N-methylglycine (Sarcosine) Treatment for Depression".
Part#ReagentTemperature
Part # 6024Enzyme Mix, 1 Vial-20C
Part # 3056Standard Curve Diluent, 40mL2-8C
Part # 4023Sarcosine Detection Reagent, 1 Vial2-8C
Part # 7021Sarcosine Standard, 1 Vial2-8C

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Cell Technology的MitoCasp同时检测线粒体膜电位和半胱天冬酶活性。读数–流式细胞仪,荧光板读数器,荧光显微镜。可靠:产生定量和定性结果。发出强烈的积极信号。该试剂盒可与其他抗体或染色剂一起使用。易于使用:无需进行细胞裂解液或蛋白质印迹分析。细胞渗透试剂。