CCND1 Split CISH Probe

CS0004

100 uL

DNA/RNA

Store at 2-8°C in an upright position. Return to storage conditions immediately after use.

CISH Probes

CCND1 Split CISH Probe is designed for the qualitative detection of human CCND1 gene at 11q13.3 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.Hybridization signals of digoxigenin-labeled polynucleotides appear dark green distinct dot-shaped (proximal to the CCND1 breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (distal to the CCND1 breakpoint region).Normal situation: In interphases of normal cells or cells without a translocation involving the CCND1 gene region, two green/red fusion signals appear.Aberrant situation: One CCND1 gene region affected by a translocation is indicated by one separate green signal and one separate red signal.Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.Unexpected signal patterns should be further investigated.

Reagent Provided:1. Digoxigenin-labeled polynucleotides, which target sequences mapping in 11q13.3* (chr11:68,522,105-68,705,283) proximal to the CCND1 breakpoint region.2. Dinitrophenyl-labeled polynucleotides, which target sequences mapping in 11q13.3* (chr11:69,453,301-70,031,240) distal to the CCND1 breakpoint region. 3. Formamide based hybridization buffer.*according to Human Genome Assembly GRCh37/hg19

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Molecular Biology > DNA Marker