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商品描述
A549 Lysate
Product Code:
PSI-1203
Pack Size:
0.1 mg
Form:
Cell Line
Target Species:
Human
Product Type:
Tissue Array
Description:
A549 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The A549 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Supplier:
ProSci
Product Category:
Tools & Reagents > Cell Lines
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- Product Information
Product Code:
PSI-1203 Price (0.1 mg):
£112.00 excluding VAT
