CLIA Kit for Alanine Aminotransferase (ALT)

SCA207Ra

96T

Sandwich CLIA Kit

CLIA

Rat

CLIA Kits

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Alanine Aminotransferase (ALT) level in the sample or standard.This assay has high sensitivity and excellent specificity for detection of Alanine Aminotransferase (ALT). No significant cross-reactivity or interference between Alanine Aminotransferase (ALT) and analogues was observed.

Detection Range: 0.14-100ng/mL The standard curve concentrations used for the CLIA?s were 100ng/mL, 33.33ng/mL, 11.11ng/mL, 3.7ng/mL, 1.23ng/mL, 0.41ng/mL, 0.14ng/mL

Sensitivity: The minimum detectable dose of this kit is typically less than0.48ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 4.5hours

Cloud-Clone Corp.

CLIA Kits