Ratprimaryantibodiesonmousetissuesareadvantageousbecauseratsecondarydetectionsystemscanbeusedwithminimumcross-reactivitytoendogenousmouseIgG.BiocareMedicalhasdevelopedaRat-on-MousehorserADIshperoxidase(HRP)polymerdetectionsystemwithminimalcross-reactivitytomousetissues.Itcanbeusedwithformalin-fixedparaffin-embeddedtissues,floatingsections,frozensectionsandcellculturepreparations.TheRat-on-MouseHRP-Polymeriscomprisedoftworeagents:ARatProbethatbindstotheratprimaryantibodyandaRat-on-MouseHRP-Polymerthatconjugatestotheprobe.Thistwo-stepsystemhasbeenproventobe10-20timesmoresensitivethanconventionalconjugatedmouseadsorbedanti-ratHRPsecondarydetectionsystems.BiocareMedicalhasnovelproductstohelpeliminatepersistentmouseIgG.XMFactorisaverypotentblockerforeliminatingendogenousmouseIgG.Byadding1-2drops(1dropequals33µl)oftheXMFactorto2.5mloftheRat-on-MousePolymer,endogenousmouseIgGwillbedramaticallyreducedand/orcompletelyeliminated.RodentBlockMisablockingreagentfornonspecificbackgroundstainingthatalsoreducesendogenousmouseIgG.RodentBlockMisappliedpriortotheprimaryantibodyfor15-30minutes.BiocareMedicalhasalsodevelopedRodentDecloaker,anantigenretrievalsolution,whichhelpsreduceand/oreliminateendogenousmouseandratIgGandnon-specificbackgroundstaining.TemperaturedependentprotocolscanbeperformedusingBiocare’sDecloakingChamber.